Recombinant Human SET And MYND Domain Containing 3, His-tagged

• Cat.No.: SMYD3-666H
• Product Overview: Recombinant Human SET And MYND Domain Containing 3 encoding the human SMYD3 isoform 1 (Met 1-Ser 428) was fused with the His tag at the C-terminus. It was Expressed inHuman Cells.

Specification

• Species: Human
• Source: Human Cells
• Tag: His
• Protein Length: 1-428 a.a.
• Description: SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains one MYND-type zinc finger and one SET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis.
• Purity: >90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70⁰C.
• Predicted N Terminal: Met.
• Molecular Mass: The recombinant human SMYD3 consists of 447 amino acids and predicts a molecular mass of 51.5 kDa. It migrates as an approxiamtely 49 KDa band in SDS-PAGE under reducing conditions
• Formulation: Supplied as a 0.2μm filtered solution of 25 mM Tris-HCl, 100 mM NaCl, 20% glycerol, 3 mM DTT, pH 8.0.
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4⁰C before opening to recover the entire contents.
• Storage: Store it under sterile conditions at -70⁰C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: SMYD3 SET and MYND domain containing 3 [ Homo sapiens ]
• Synonyms: SET and MYND domain containing 3; KMT3E; ZMYND1; ZNFN3A1; FLJ21080; MGC104324; bA74P14.1; SMYD3; bA74P14.1 (novel protein); zinc finger, MYND domain containing 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; EC 2.1.1.43; Zinc finger MYND domain-containing protein 1
• Gene ID: 64754
• mRNA Refseq: NM_001167740
• Protein Refseq: NP_001161212
• MIM: 608783
• UniProt ID: Q9H7B4
• Chromosome Location: 1q44
• Function: histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; protein binding; transferase activity; zinc ion binding