Recombinant Human SMYD3 protein(Lys35-Ser369), GST-tagged

• Cat.No.: SMYD3-920H
• Product Overview: Recombinant Human SMYD3 isoform 2 (NP_073580.1) (Lys 35-Ser 369) was expressed in Insect Cells, fused with the GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 35-369 a.a.
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.5mM DTT, 0.5mM GSH, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human SMYD3/GST chimera consists of 559 amino acids and predicts a molecular mass of 656 kDa. It migrates as an approxiamtely 58 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SMYD3 SET and MYND domain containing 3 [ Homo sapiens ]
• Official Symbol: SMYD3
• Synonyms: SMYD3; SET and MYND domain containing 3; zinc finger, MYND domain containing 1 , ZMYND1, ZNFN3A1; SET and MYND domain-containing protein 3; KMT3E; bA74P14.1 (novel protein); zinc finger, MYND domain containing 1; zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; ZMYND1; ZNFN3A1; bA74P14.1; FLJ21080; MGC104324
• Gene ID: 64754
• mRNA Refseq: NM_001167740
• Protein Refseq: NP_001161212
• MIM: 608783
• UniProt ID: Q9H7B4