How is IL16's chemotactic activity assessed in leukocyte migration assays?
Leukocyte migration assays measure IL16's chemotactic impact on cells.
What methods investigate IL16's modulation of T cell activation markers?
Flow cytometry and qPCR assess T cell activation markers affected by IL16.
How do gene expression arrays unveil IL16's role in inflammatory responses?
Gene expression arrays reveal IL16's influence on inflammatory gene profiles.
Could you detail the use of RNA interference in knocking down IL16 expression?
RNA interference used to knock down IL16 expression in cell lines.
Can you explain the experimental setup for studying IL16's impact on HIV infection?
Experimental setup for studying IL16's modulation of HIV infection involves viral assays.
What techniques determine IL16's binding affinity to CD4 receptors?
Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.
How are flow cytometry and confocal microscopy utilized to examine IL16's localization?
Flow cytometry and confocal microscopy visualize IL16's cellular localization.
What experimental models aid in deciphering IL16's role in autoimmune disorders?
Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.
Could you elaborate on the use of ELISA in quantifying IL16 secretion?
ELISA quantifies secreted IL16 levels from cell culture supernatants.
What strategies discern IL16's involvement in allergic diseases?
Strategies like animal models and patient samples explore IL16's role in allergies.