Active Recombinant Mouse Interleukin 16

Cat.No. : Il16-486M
Product Overview : Mouse IL-16 is comprised of 127 amino acids with a molecular weight of 13.2 kDa
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Cat. No. : Il16-486M
Description : Mouse IL-16 was originally described as Lymphocyte Chemoattractant Factor (LCF), is produced primarily by CD4 (+) and CD8 (+) T cells and acts as a chemoattractant for lymphocytes, monocytes, eosinophils, dendritic cells and Langerhanscells. Additionally, IL-16 has been reported to upregulate IL-2 receptor (CD25), induce progression of cells to the G (1) phase and suppresses HIV & SIV replication. Other cells which have been shown to express IL-16 are; B-cells, eosinophils, macrophages, epithelial cells, fibroblasts, microglial cells and keratinocytes. Human and Mouse IL-16 share a high degree of structural and functional similarity.
Species : Mouse
Source : E.coli
Form : Lyophilized at a concentration of 1 mg/ml in 10mM NaP, PH 7.5
Purity : Purity Greater than 97% as determined by: Reducing and non-reducing SDS-PAGE.
Bio-activity : Recombinant mouse IL-16 is fully biologically active compared to standard. The ED50, as determined by dose-dependent chemotactic induction of mouse CD4+ T-cells, is 200ng/ml.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mg"s/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 97% as determined by : Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence : MHDLNSSTDS AASASAASDI SVESKEATVC TVTLEKTSAG LGFSLEGGKG SLHGDKPLTI NRIFKGDRTGEMVQPGDEIL QLAGTAVQGL TRFEAWNVIK ALPDGPVTIV IRRTSLQCKQ TTASADS
Storage : Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
SDS-PAGE:
Gene Name : Il16 interleukin 16 [ Mus musculus ]
Official Symbol : Il16
Synonyms : Il16; interleukin 16; KIAA4048; mKIAA4048
Gene ID : 16170
mRNA Refseq : NM_010551
Protein Refseq : NP_034681
UniProt ID : O54824
Chromosome Location : 7 D2-D3; 7 41.2 cM
Function : cytokine activity; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How is IL16's chemotactic activity assessed in leukocyte migration assays? 12/10/2022

Leukocyte migration assays measure IL16's chemotactic impact on cells.

What methods investigate IL16's modulation of T cell activation markers? 09/26/2022

Flow cytometry and qPCR assess T cell activation markers affected by IL16.

How do gene expression arrays unveil IL16's role in inflammatory responses? 05/18/2022

Gene expression arrays reveal IL16's influence on inflammatory gene profiles.

Could you detail the use of RNA interference in knocking down IL16 expression? 01/17/2022

RNA interference used to knock down IL16 expression in cell lines.

Can you explain the experimental setup for studying IL16's impact on HIV infection? 07/25/2021

Experimental setup for studying IL16's modulation of HIV infection involves viral assays.

What techniques determine IL16's binding affinity to CD4 receptors? 10/12/2019

Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.

How are flow cytometry and confocal microscopy utilized to examine IL16's localization? 12/06/2018

Flow cytometry and confocal microscopy visualize IL16's cellular localization.

What experimental models aid in deciphering IL16's role in autoimmune disorders? 07/24/2017

Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.

Could you elaborate on the use of ELISA in quantifying IL16 secretion? 05/16/2017

ELISA quantifies secreted IL16 levels from cell culture supernatants.

What strategies discern IL16's involvement in allergic diseases? 01/30/2017

Strategies like animal models and patient samples explore IL16's role in allergies.

Customer Reviews (3)

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Reviews
08/31/2021

    Suitable for ELISA.

    06/28/2019

      High specificity in ELISA.

      02/05/2018

        Potent in chemotaxis studies.

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