Recombinant Human IL16 protein, His-tagged

• Cat.No.: IL16-551H

Specification

• Product overview: Recombinant Human IL16 (Q14005) aa. (Met1203~Ser1332) fused with His tag was produced in E. coli cells.
• Description: The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 17.1kDa; Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met1203~Ser1332
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Characteristic: Isoelectric point is 6.
• Applications: Cell culture; Activity Assays; In vivo assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: IL16 interleukin 16 [ Homo sapiens (human) ]
• Official Symbol: IL16
• Synonyms: IL16; interleukin 16; LCF; NIL16; PRIL16; prIL-16; pro-interleukin-16; lymphocyte chemoattractant factor; neuronal interleukin 16; prointerleukin 16
• Gene ID: 3603
• mRNA Refseq: NM_001172128.1
• Protein Refseq: NP_001165599.1
• UniProt ID: Q14005

Reviews

08/31/2021

Suitable for ELISA.

06/28/2019

High specificity in ELISA.

02/05/2018

Potent in chemotaxis studies.

Q&As

How is IL16's chemotactic activity assessed in leukocyte migration assays?

Leukocyte migration assays measure IL16's chemotactic impact on cells.

What methods investigate IL16's modulation of T cell activation markers?

Flow cytometry and qPCR assess T cell activation markers affected by IL16.

How do gene expression arrays unveil IL16's role in inflammatory responses?

Gene expression arrays reveal IL16's influence on inflammatory gene profiles.

Could you detail the use of RNA interference in knocking down IL16 expression?

RNA interference used to knock down IL16 expression in cell lines.

Can you explain the experimental setup for studying IL16's impact on HIV infection?

Experimental setup for studying IL16's modulation of HIV infection involves viral assays.

What techniques determine IL16's binding affinity to CD4 receptors?

Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.

How are flow cytometry and confocal microscopy utilized to examine IL16's localization?

Flow cytometry and confocal microscopy visualize IL16's cellular localization.

What experimental models aid in deciphering IL16's role in autoimmune disorders?

Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.

Could you elaborate on the use of ELISA in quantifying IL16 secretion?

ELISA quantifies secreted IL16 levels from cell culture supernatants.

What strategies discern IL16's involvement in allergic diseases?

Strategies like animal models and patient samples explore IL16's role in allergies.