Recombinant Full Length Human IL16 Protein, C-Flag-tagged

• Cat.No.: IL16-1322HFL
• Product Overview: Recombinant Full Length Human IL16 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 66.5 kDa
• AA Sequence: MDYSFDTTAEDPWVRISDCIKNLFS PIMSENHGHMPLQPNASLNEEEGTQ GHPDGTPPKLDTANGTPKVY KSADSSTVKKGPPVAPKPAWFRQSL KGLRNRASDPRGLPDPALSTQPAPA SREHLGSHIRASSSSSSIRQ RISSFETFGSSQLPDKGAQRLSLQP SSGEAAKPLGKHEEGRFSGLLGRGA APTLVPQQPEQVLSSGSPAA SEARDPGVSESPPPGRQPNQKTLPP GPDPLLRLLSTQAEESQGPVLKMPS QRARSFPLTRSQSCETKLLD EKTSKLYSISSQVSSAVMKSLLCLP SSISCAQTPCIPKEGASPTSSSNED SAANGSAETSALDTGFSLNL SELREYTEGLTEAKEDDDGDHSSLQ SGQSVISLLSSEELKKLIEEVKVLD EATLKQLDGIHVTILHKEEG AGLGFSLAGGADLENKVITVHRVFP NGLASQEGTIQKGNEVLSINGKSLK GTTHHDALAILRQAREPRQA VIVTRKLTPEAMPDLNSSTDSAASA SAASDVSVESTAEATVCTVTLEKMS AGLGFSLEGGKGSLHGDKPL TINRIFKGAASEQSETVQPGDEILQ LGGTAMQGLTRFEAWNIIKALPDGP VTIVIRRKSLQSKETTAAGD
  STRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein

Gene Information

• Gene Name: IL16 interleukin 16 [ Homo sapiens (human) ]
• Official Symbol: IL16
• Synonyms: LCF; NIL16; PRIL16; prIL-16
• Gene ID: 3603
• mRNA Refseq: NM_004513.6
• Protein Refseq: NP_004504.3
• MIM: 603035
• UniProt ID: Q9UME6

Reviews

08/31/2021

Suitable for ELISA.

06/28/2019

High specificity in ELISA.

02/05/2018

Potent in chemotaxis studies.

Q&As

How is IL16's chemotactic activity assessed in leukocyte migration assays?

Leukocyte migration assays measure IL16's chemotactic impact on cells.

What methods investigate IL16's modulation of T cell activation markers?

Flow cytometry and qPCR assess T cell activation markers affected by IL16.

How do gene expression arrays unveil IL16's role in inflammatory responses?

Gene expression arrays reveal IL16's influence on inflammatory gene profiles.

Could you detail the use of RNA interference in knocking down IL16 expression?

RNA interference used to knock down IL16 expression in cell lines.

Can you explain the experimental setup for studying IL16's impact on HIV infection?

Experimental setup for studying IL16's modulation of HIV infection involves viral assays.

What techniques determine IL16's binding affinity to CD4 receptors?

Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.

How are flow cytometry and confocal microscopy utilized to examine IL16's localization?

Flow cytometry and confocal microscopy visualize IL16's cellular localization.

What experimental models aid in deciphering IL16's role in autoimmune disorders?

Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.

Could you elaborate on the use of ELISA in quantifying IL16 secretion?

ELISA quantifies secreted IL16 levels from cell culture supernatants.

What strategies discern IL16's involvement in allergic diseases?

Strategies like animal models and patient samples explore IL16's role in allergies.