Recombinant Human IL16 Protein (Met1203-Ser1332), C-His tagged, Animal-free, Carrier-free

• Cat.No.: IL16-181H
• Product Overview: Recombinant Human IL16 Protein (Met1203-Ser1332) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin 16(IL-16) predicts a molecular mass of 13.5 kDa, encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication, and signaling process of this cytokine is mediated by CD4.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 14.12 kDa.
  The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Met1203-Ser1332
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: IL16 interleukin 16 [ Homo sapiens (human) ]
• Official Symbol: IL16
• Synonyms: IL16; interleukin 16; interleukin 16 (lymphocyte chemoattractant factor); pro-interleukin-16; FLJ16806; FLJ42735; HsT19289; IL 16; LCF; lymphocyte chemoattractant factor; prIL 16; prointerleukin 16; neuronal interleukin 16; NIL16; PRIL16; prIL-16; FLJ44234
• Gene ID: 3603
• mRNA Refseq: NM_001172128
• Protein Refseq: NP_001165599
• MIM: 603035
• UniProt ID: Q14005

Reviews

08/31/2021

Suitable for ELISA.

06/28/2019

High specificity in ELISA.

02/05/2018

Potent in chemotaxis studies.

Q&As

How is IL16's chemotactic activity assessed in leukocyte migration assays?

Leukocyte migration assays measure IL16's chemotactic impact on cells.

What methods investigate IL16's modulation of T cell activation markers?

Flow cytometry and qPCR assess T cell activation markers affected by IL16.

How do gene expression arrays unveil IL16's role in inflammatory responses?

Gene expression arrays reveal IL16's influence on inflammatory gene profiles.

Could you detail the use of RNA interference in knocking down IL16 expression?

RNA interference used to knock down IL16 expression in cell lines.

Can you explain the experimental setup for studying IL16's impact on HIV infection?

Experimental setup for studying IL16's modulation of HIV infection involves viral assays.

What techniques determine IL16's binding affinity to CD4 receptors?

Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.

How are flow cytometry and confocal microscopy utilized to examine IL16's localization?

Flow cytometry and confocal microscopy visualize IL16's cellular localization.

What experimental models aid in deciphering IL16's role in autoimmune disorders?

Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.

Could you elaborate on the use of ELISA in quantifying IL16 secretion?

ELISA quantifies secreted IL16 levels from cell culture supernatants.

What strategies discern IL16's involvement in allergic diseases?

Strategies like animal models and patient samples explore IL16's role in allergies.