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Recombinant Human IL16 protein

Cat.No. : IL16-503H
Product Overview : Recombinant Human IL16 protein (121 a.a.) was expressed in Escherichia coli.
  • Specification
  • Gene Information
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Description : Human Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it has three isoforms which are cleaved from pre-Interleukin-16. IL-16 is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant human IL-16 (121 a.a.) contains 121 amino acid residues and it shares 85 % a.a. sequence identity with murine IL-16.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T Lymphocytes is in a concentration range of 50-500 ng/ml.
Molecular Mass : Approximately 12.4 kDa, a single non-glycosylated polypeptide chain containing 121 amino acids.
Protein length : 121
AA Sequence : SAASASAASDVSVESTAEATVCTVT LEKMSAGLGFSLEGGKGSLHGDKPL TINRIFKGAASEQSETVQPGDEILQ LGGTAMQGLTRFEAWNIIKALPDGP VTIVIRRKSLQSKETTAAGDS
Endotoxin : Less than 1 EU/µg of rHuIL-16, 121 a.a. as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : IL16
Official Symbol : IL16
Synonyms : IL16; interleukin 16; interleukin 16 (lymphocyte chemoattractant factor); pro-interleukin-16; FLJ16806; FLJ42735; HsT19289; IL 16; LCF; lymphocyte chemoattractant factor; prIL 16; prointerleukin 16; neuronal interleukin 16; NIL16; PRIL16; prIL-16; FLJ44234;
Gene ID : 3603
mRNA Refseq : NM_001172128
Protein Refseq : NP_001165599
MIM : 603035
UniProt ID : Q14005

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How is IL16's chemotactic activity assessed in leukocyte migration assays? 12/10/2022

Leukocyte migration assays measure IL16's chemotactic impact on cells.

What methods investigate IL16's modulation of T cell activation markers? 09/26/2022

Flow cytometry and qPCR assess T cell activation markers affected by IL16.

How do gene expression arrays unveil IL16's role in inflammatory responses? 05/18/2022

Gene expression arrays reveal IL16's influence on inflammatory gene profiles.

Could you detail the use of RNA interference in knocking down IL16 expression? 01/17/2022

RNA interference used to knock down IL16 expression in cell lines.

Can you explain the experimental setup for studying IL16's impact on HIV infection? 07/25/2021

Experimental setup for studying IL16's modulation of HIV infection involves viral assays.

What techniques determine IL16's binding affinity to CD4 receptors? 10/12/2019

Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.

How are flow cytometry and confocal microscopy utilized to examine IL16's localization? 12/06/2018

Flow cytometry and confocal microscopy visualize IL16's cellular localization.

What experimental models aid in deciphering IL16's role in autoimmune disorders? 07/24/2017

Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.

Could you elaborate on the use of ELISA in quantifying IL16 secretion? 05/16/2017

ELISA quantifies secreted IL16 levels from cell culture supernatants.

What strategies discern IL16's involvement in allergic diseases? 01/30/2017

Strategies like animal models and patient samples explore IL16's role in allergies.

Customer Reviews (3)

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Reviews
08/31/2021

    Suitable for ELISA.

    06/28/2019

      High specificity in ELISA.

      02/05/2018

        Potent in chemotaxis studies.

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