Recombinant Mouse Il16 Protein, MYC/DDK-tagged

• Cat.No.: Il16-1239M
• Product Overview: Purified recombinant protein of full-length mouse interleukin 16 (Il16), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Description: Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 141.4 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Il16 interleukin 16 [ Mus musculus (house mouse) ]
• Official Symbol: Il16
• Synonyms: Il16; interleukin 16; mKIAA4048; pro-interleukin-16
• Gene ID: 16170
• mRNA Refseq: NM_010551
• Protein Refseq: NP_034681
• UniProt ID: O54824

Reviews

08/31/2021

Suitable for ELISA.

06/28/2019

High specificity in ELISA.

02/05/2018

Potent in chemotaxis studies.

Q&As

How is IL16's chemotactic activity assessed in leukocyte migration assays?

Leukocyte migration assays measure IL16's chemotactic impact on cells.

What methods investigate IL16's modulation of T cell activation markers?

Flow cytometry and qPCR assess T cell activation markers affected by IL16.

How do gene expression arrays unveil IL16's role in inflammatory responses?

Gene expression arrays reveal IL16's influence on inflammatory gene profiles.

Could you detail the use of RNA interference in knocking down IL16 expression?

RNA interference used to knock down IL16 expression in cell lines.

Can you explain the experimental setup for studying IL16's impact on HIV infection?

Experimental setup for studying IL16's modulation of HIV infection involves viral assays.

What techniques determine IL16's binding affinity to CD4 receptors?

Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.

How are flow cytometry and confocal microscopy utilized to examine IL16's localization?

Flow cytometry and confocal microscopy visualize IL16's cellular localization.

What experimental models aid in deciphering IL16's role in autoimmune disorders?

Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.

Could you elaborate on the use of ELISA in quantifying IL16 secretion?

ELISA quantifies secreted IL16 levels from cell culture supernatants.

What strategies discern IL16's involvement in allergic diseases?

Strategies like animal models and patient samples explore IL16's role in allergies.