Recombinant Mouse Il16 Protein, MYC/DDK-tagged

Cat.No. : Il16-1239M
Product Overview : Purified recombinant protein of full-length mouse interleukin 16 (Il16), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.
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Description : Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
Source : HEK293T
Species : Mouse
Tag : MYC/DDK
Molecular Mass : 141.4 kDa
Purity : >80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 µg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Il16 interleukin 16 [ Mus musculus (house mouse) ]
Official Symbol : Il16
Synonyms : Il16; interleukin 16; mKIAA4048; pro-interleukin-16
Gene ID : 16170
mRNA Refseq : NM_010551
Protein Refseq : NP_034681
UniProt ID : O54824

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How is IL16's chemotactic activity assessed in leukocyte migration assays? 12/10/2022

Leukocyte migration assays measure IL16's chemotactic impact on cells.

What methods investigate IL16's modulation of T cell activation markers? 09/26/2022

Flow cytometry and qPCR assess T cell activation markers affected by IL16.

How do gene expression arrays unveil IL16's role in inflammatory responses? 05/18/2022

Gene expression arrays reveal IL16's influence on inflammatory gene profiles.

Could you detail the use of RNA interference in knocking down IL16 expression? 01/17/2022

RNA interference used to knock down IL16 expression in cell lines.

Can you explain the experimental setup for studying IL16's impact on HIV infection? 07/25/2021

Experimental setup for studying IL16's modulation of HIV infection involves viral assays.

What techniques determine IL16's binding affinity to CD4 receptors? 10/12/2019

Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.

How are flow cytometry and confocal microscopy utilized to examine IL16's localization? 12/06/2018

Flow cytometry and confocal microscopy visualize IL16's cellular localization.

What experimental models aid in deciphering IL16's role in autoimmune disorders? 07/24/2017

Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.

Could you elaborate on the use of ELISA in quantifying IL16 secretion? 05/16/2017

ELISA quantifies secreted IL16 levels from cell culture supernatants.

What strategies discern IL16's involvement in allergic diseases? 01/30/2017

Strategies like animal models and patient samples explore IL16's role in allergies.

Customer Reviews (3)

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Reviews
08/31/2021

    Suitable for ELISA.

    06/28/2019

      High specificity in ELISA.

      02/05/2018

        Potent in chemotaxis studies.

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