Active GMP Recombinant Human NOG protein

• Cat.No.: NOG-119HG
• Product Overview: GMP Recombinant Human NOG protein(NP_005441.1)(211 aa), fused with His Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: HEK293
• Protein Length: DNA sequence encoding Human noggin(NP_005441.1) was expressed with His tag at the C-terminal.
• Bio-activity: Measured by its ability to inhibit 5.0ng/ml BMP-4-induced alkaline phosphatase production by ATDC chondrocytes. The ED50 for this effect is 2.0-3.0ng/ml in the presence of 2.0-3.0ng/ml of Noggin.
• Molecular Mass: Recombinant Human NOG contains 211 aa. It has a predicted MW of 23.9 kDa.
• Endotoxin: <=0.01EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NOG noggin [ Homo sapiens ]
• Official Symbol: NOG
• Synonyms: Noggin; NOG; SYM1; SYNS1
• Gene ID: 9241
• mRNA Refseq: NM_005450
• Protein Refseq: NP_005441
• MIM: 602991
• UniProt ID: Q13253