Recombinant Human NOG protein, Fc-tagged, For Organoid Culture

• Cat.No.: NOG-3827H
• Product Overview: Recombinant Human NOG protein(Q13253)(Gln28-Cys232), fused with C-terminal Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Gln28-Cys232
• Tag: C-Fc
• Form: Lyophilized from sterile PBS, pH 7.4, 3% trehalose.
• Bio-activity: Recombinant human Noggin Fc chimera protein inhibits BMP2 inducted luciferase expression in the NIH-3T3-BRE, which is a BMP responsive reporter cell line. The luciferase expression was analyzed using the Bright-Lite Luciferase Assay System (Vazyme Biotech). The ED50 for this effect is 0.100-0.500 μg/mL in the presence of 10ng/mL of recombinant human BMP-2.
• Molecular Mass: 49.4 kDa (monomer)
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions.
• Storage: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below: To the date of expiration, -20°C to -80°C as supplied. 3 months, -20°C to -80°C under sterile conditions after reconstitution. 1 month, 2 to 8 °C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NOG noggin [ Homo sapiens ]
• Official Symbol: NOG
• Synonyms: NOG; noggin; SYM1, symphalangism 1 (proximal) , synostoses (multiple) syndrome 1 , SYNS1; symphalangism 1 (proximal); SYM1; SYNS1
• Gene ID: 9241
• mRNA Refseq: NM_005450
• Protein Refseq: NP_005441
• MIM: 602991
• UniProt ID: Q13253