Recombinant Human NOG protein(Met1-Cys232), hFc-tagged

• Cat.No.: NOG-817H
• Product Overview: Recombinant Human NOG (NP_005441.1) (Met 1-Cys 232) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Protein length: Met1-Cys232
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.1-0.6 μg/mL in the presence of 25 ng/mL of hBMP4.
• Molecular Mass: The recombinant human Noggin/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. Each monomer comprises 443 amino acids and has a predicted molecular mass of 49.8 kDa. As a result of glycosylation, the apparent molecular mass of rhNoggin/Fc monomer is approximately 58-62 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NOG noggin [ Homo sapiens ]
• Official Symbol: NOG
• Synonyms: NOG; noggin; SYM1, symphalangism 1 (proximal) , synostoses (multiple) syndrome 1 , SYNS1; symphalangism 1 (proximal); SYM1; SYNS1
• Gene ID: 9241
• mRNA Refseq: NM_005450
• Protein Refseq: NP_005441
• MIM: 602991
• UniProt ID: Q13253

Reviews

07/15/2022

Efficient peptide synthesis, accelerates our peptide research projects.

04/30/2018

Fast and reliable protein expression, aids our experiments significantly.

01/25/2018

Dependable antibody validation, crucial for our immunoprecipitation studies.

Q&As

How does NOG (Noggin) modulate bone morphogenetic protein (BMP) signaling in development and tissue repair?

NOG inhibits BMP signaling, critical for tissue development and repair processes.

What are the mechanisms by which NOG modulates embryonic patterning and organogenesis?

NOG modulates embryonic patterning and organogenesis, guiding proper organ development.

How do alterations in NOG expression or function affect the development of congenital anomalies?

Changes in NOG expression or function can lead to congenital anomalies due to disrupted developmental processes.

How does NOG contribute to the regulation of stem cell differentiation?

NOG regulates stem cell differentiation, influencing their development into various tissue types.

What role does NOG play in skeletal development and the regulation of bone growth?

NOG plays a key role in skeletal development by regulating bone growth and differentiation.

How does NOG influence neural development and neurogenesis?

NOG influences neural development, impacting the formation and specialization of neural cells.

What is the impact of NOG in joint and cartilage health, particularly in arthritis?

NOG is important for joint and cartilage health, potentially impacting arthritis progression.