| Description : |
Noggin, also known as NOG, is a homodimeric glycoprotein that binds to and modulates the activity of TGF-beta family ligands. It is expressed in condensing cartilage and immature chondrocytes. Noggin antagonizes bone morphogenetic protein (BMP) activities by blocking epitopes on BMPs needed for binding to their receptors.Noggin has been shown to be involved in many developmental processes, such as neural tube formation and joint formation. During development, Noggin diffuses through extracellular matrices and forms morphogenic gradients that regulate cellular responses in a concentration-dependent manner. |
| Source : |
CHO |
| Species : |
Mouse |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50< 60 ng/mL, measured in a bioassay using ATDC5 cells in the presence of 10 ng/mL human BMP-4. |
| Molecular Mass : |
29-31 kDa, observed by reducing SDS-PAGE. |
| AA Sequence : |
LRAAPAGGQHYLHIRPAPSDNLPLV DLIEHPDPIFDPKEKDLNETLLRSL LGGHYDPGFMATSPPEDRPGGGGGP AGGAEDLAELDQLLRQRPSGAMPSE IKGLEFSEGLAQGKKQRLSKKLRRK LQMWLWSQTFCPVLYAWNDLGSRFW PRYVKVGSCFSKRSCSVPEGMVCKP SKSVHLTVLRWRCQRRGGQRCGWIP IQYPIISECKCSC |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE. |
| Storage : |
Lyophilized recombinant murine Nogginremains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, murine Nogginshould be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
