Species : |
Human |
Source : |
HEK293 |
Tag : |
Non |
Protein Length : |
Gln28-Cys232 |
Tag : |
Non |
Form : |
Lyophilized from sterile PBS, pH 7.4, 3% trehalose. |
Bio-activity : |
Recombinant human Noggin protein inhibits BMP2 inducted luciferase expression in the NIH-3T3-BRE, which is a BMP responsive reporter cell line. The luciferase expression was analyzed using the Bright-Lite Luciferase Assay System (Vazyme Biotech). The ED50 for this effect is 20-100 ng/mL in the presence of 10ng/mL of recombinant human BMP-2. |
Molecular Mass : |
28 kDa (monomer) |
Endotoxin : |
<0.10 EU/μg of the protein by the LAL method. |
Purity : |
>90%, by SDS-PAGE under reducing conditions. |
Storage : |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below: To the date of expiration, -20°C to -80°C as supplied. 3 months, -20°C to -80°C under sterile conditions after reconstitution. 1 month, 2 to 8 °C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |