NRP1

  • Official Full Name

    neuropilin 1

  • Overview

    This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
  • Synonyms

    NRP1; neuropilin 1; NP1; NRP; BDCA4; CD304; VEGF165R; neuropilin-1; transmembrane receptor; vascular endothelial cell growth factor 165 receptor;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Cynomolgus
  • Cynomolgus Monkey
  • Human
  • Mouse
  • Rat
  • Rhesus Macaque
  • E.coli
  • HEK293
  • HEK293F
  • HEK293T
  • Human Cell
  • Mammalian Cell
  • Mammalian cells
  • C
  • Fc
  • hFc
  • His
  • Avi
  • mFc
  • Fc|His
  • His (Fc)
  • His|GST
  • His|MBP
  • human|IgG1|Fc
  • Mouse|IgG2a|Fc
  • Myc
  • DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human NRP1-644H Active Recombinant Human NRP1 protein(Met1-Lys644), His-tagged HEK293 C-His Met1-Lys644
Human NRP1-090H Active Recombinant Human NRP1 Protein(Met 1-Lys 644, with natural variants V179A), His-tagged HEK293 His Met 1-Lys 644, with natural variants V179A
Human NRP1-1365H Acitve Recombinant Human NRP1 protein(Met1-Lys644), His&Avi-tagged, Biotinylated HEK293 C-His&Avi Met1-Lys644
Human NRP1-803H Recombinant Human NRP1 protein, His-tagged E.coli His Tyr646~Asp814 (Accession # O14786)
Human NRP1-3346H Recombinant Human NRP1 protein, Fc-tagged HEK293 human/IgG1/Fc Phe22-Lys644
Human NRP1-804H Recombinant Human NRP1 protein, His-tagged E.coli His Cys27~Arg143 (Accession # O14786)
Human NRP1-1763HCL Recombinant Human NRP1 cell lysate Human Cell N/A
Human NRP1-806H Recombinant Human NRP1 protein, His-tagged E.coli His
Human NRP1-396H Recombinant Human NRP1 Protein, His-tagged HEK293 His 923
Human NRP1-1321H Recombinant Human NRP1 Protein (Lys274-Glu584), N-His tagged E.coli N-His Lys274-Glu584
Human NRP1-3684H Recombinant Human NRP1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human NRP1-0566H Active Recombinant Human NRP1 protein, Fc-tagged HEK293 Fc Phe22-Lys644
Human NRP1-3684H-B Recombinant Human NRP1 Protein Pre-coupled Magnetic Beads HEK293
Human NRP1-3752H Recombinant Human NRP1 Protein (Met1-Lys644), C-Fc tagged Mammalian cells C-Fc Met1-Lys644
Human NRP1-2512H Recombinant Human NRP1 Protein, His-tagged E.coli N-His Cys27-Asp444
Human NRP1-2644H Recombinant Human NRP1 Protein (Phe22-Lys644), His tagged E.coli His Phe22-Lys644
Human NRP1-5255H Recombinant Human NRP1 Protein (Phe22-Lys644), C-His tagged Mammalian cells C-His Phe22-Lys644
Human NRP1-5254H Recombinant Human NRP1 Protein (Met1-Lys644), C-His tagged Mammalian cells C-His Met1-Lys644
Human NRP1-1322H Recombinant Human NRP1 Protein (Phe22-Ser641), N-His tagged E.coli N-His Phe22-Ser641
Human NRP1-2065H Recombinant Human NRP1 protein, His-tagged HEK293 His Phe22-Lys644
Human NRP1-8119H Active Recombinant Human NRP1 protein, His-Avi-tagged, Biotinylated HEK293 His-Avi Phe 22 - Lys 644
Human NRP1-5594H Recombinant Human NRP1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human NRP1-805H Recombinant Human NRP1 protein, His-tagged E.coli His Cys27-Asp444
Human NRP1-3347H Active Recombinant Human NRP1 protein, mFc-tagged HEK293 mFc Phe 22 - Lys 644
Human NRP1-159H Recombinant Human NRP1 Protein, His-tagged E.coli His
Human NRP1-1159H Recombinant Human NRP1 Protein, His-tagged HEK293F N-His Cys27-Asp444
Human NRP1-397H Recombinant Human NRP1 Protein, Fc-tagged HEK293 Fc 923
Mouse Nrp1-613M Active Recombinant Mouse Nrp1 Protein, His-tagged Mammalian cells His Phe22-Pro856
Mouse Nrp1-807M Recombinant Mouse Nrp1 protein, His-tagged E.coli His Cys275~Cys424 (Accession # P97333)
Mouse Nrp1-805M Recombinant Mouse Nrp1 protein, His-tagged E.coli His Cys27~Phe141 (Accession # P97333)
Mouse NRP1-10898M Recombinant Mouse NRP1 Protein Mammalian Cell His
Mouse Nrp1-808M Recombinant Mouse Nrp1 protein, His-tagged E.coli His Cys431~Cys583 (Accession # P97333)
Mouse Nrp1-806M Recombinant Mouse Nrp1 protein, His-tagged E.coli His Cys147~Leu265 (Accession # P97333)
Mouse NRP1-329M Recombinant Mouse NRP1 protein, Mouse IgG2a Fc-tagged, low endotoxin HEK293 Mouse/IgG2a/Fc 835
Mouse Nrp1-4502M Recombinant Mouse Nrp1 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse Nrp1-8118M Active Recombinant Mouse Nrp1 protein(Met1-Pro856), mFc-tagged HEK293 C-mFc Met1-Pro856
Mouse NRP1-680M Active Recombinant Mouse NRP1 protein, mFc-tagged HEK293 mFc Phe 22 - Pro 856
Mouse NRP1-0565M Active Recombinant Mouse NRP1 protein(Phe22-Pro856), His-tagged HEK293 C-His Phe22-Pro856
Mouse NRP1-6210M Recombinant Mouse NRP1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse NRP1-6210M-B Recombinant Mouse NRP1 Protein Pre-coupled Magnetic Beads HEK293
Mouse Nrp1-6211M Recombinant Mouse Nrp1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Nrp1-6211M-B Recombinant Mouse Nrp1 Protein Pre-coupled Magnetic Beads HEK293
Rat NRP1-4084R Recombinant Rat NRP1 Protein Mammalian Cell His
Rat Nrp1-810R Recombinant Rat Nrp1 protein, His-tagged E.coli His Asn150~Val264 (Accession # Q9QWJ9)
Rat Nrp1-812R Recombinant Rat Nrp1 protein, His & MBP-tagged E.coli His/MBP Cys27~Thr152 (Accession # Q9QWJ9)
Rat Nrp1-809R Recombinant Rat Nrp1 protein, His-tagged E.coli His Thr645~Glu821 (Accession # Q9QWJ9)
Rat Nrp1-811R Recombinant Rat Nrp1 protein, His & GST-tagged E.coli His/GST Cys27~Thr152 (Accession # Q9QWJ9)
Rat NRP1-3743R Recombinant Rat NRP1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat NRP1-3743R-B Recombinant Rat NRP1 Protein Pre-coupled Magnetic Beads HEK293
Cynomolgus NRP1-1218C Active Recombinant Cynomolgus NRP1 protein(Met1-Gly613), His-tagged HEK293 C-His Met1-Gly613
Cynomolgus NRP1-1290C Acitve Recombinant Cynomolgus NRP1 protein(Met1-Gly613), hFc-tagged HEK293 C-hFc Met1-Gly613
Cynomolgus NRP1-1219C Active Recombinant Cynomolgus NRP1 protein HEK293 N/A Met1-Gly613
Cynomolgus Monkey NRP1-157C Recombinant Cynomolgus NRP1, Fc tagged Human Cell Fc/His
Cynomolgus Monkey NRP1-158C Recombinant Cynomolgus NRP1, LEVLFQ tagged Human Cell His
Cynomolgus Monkey NRP1-811CCL Recombinant Cynomolgus NRP1 cell lysate Human Cell N/A
Rhesus Macaque NRP1-3104R Recombinant Rhesus monkey NRP1 Protein, His-tagged Mammalian Cell His
Rhesus Macaque NRP1-2923R Recombinant Rhesus Macaque NRP1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque NRP1-2923R-B Recombinant Rhesus Macaque NRP1 Protein Pre-coupled Magnetic Beads HEK293
Chicken NRP1-6346C Recombinant Chicken NRP1 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is NRP1 protein?

NRP1 (neuropilin 1) gene is a protein coding gene which situated on the short arm of chromosome 10 at locus 10p11. This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. The NRP1 protein is consisted of 923 amino acids and its molecular mass is approximately 103.1 kDa.

What is the function of NRP1 protein?

NRP1 protein is a multifunctional membrane bound glycoprotein that plays a key role in the nervous system, cardiovascular system and tumorigenesis. NRP1, as a co-receptor of neuroguidance factors, is involved in axon guidance and the formation of neural circuits. During angiogenesis, NRP1 regulates endothelial cell migration and vascular stability by interacting with VEGF and other factors. In addition, NRP1 also plays an important role in tumor growth, invasion and metastasis, promoting tumor angiogenesis and cell migration by binding to its ligand.

NRP1 Related Signaling Pathway

The signaling pathways involved in NRP1 protein include axon homing, neural circuit formation, angiogenesis and tumorigenesis. In the nervous system, NRP1 acts as a co-receptor interacting with a variety of neuro-directing factors to regulate axon growth and orientation. In the cardiovascular system, NRP1 is involved in the formation and stabilization of blood vessels by binding to factors such as VEGF. In addition, NRP1 also plays an important role in tumor growth, invasion and metastasis, promoting tumor angiogenesis and cell migration by binding to its ligand.

NRP1-9.jpg

Fig1. Neuropilin-1-triggered signaling pathways in the tumor vasculature and the tumor microenvironment. (Stephan Niland, 2019)

NRP1 Related Diseases

The NRP1 protein has been implicated in a variety of diseases, mainly including neurological diseases, cardiovascular diseases, and tumors. In the nervous system, abnormal expression of NRP1 may lead to neurodevelopmental disorders and neurodegenerative diseases. In the cardiovascular system, NRP1 is involved in the regulation of angiogenesis and vascular stability, and its abnormality may lead to cardiovascular diseases such as atherosclerosis. In addition, NRP1 is highly expressed in a variety of tumors and affects tumor growth, invasion and metastasis by promoting tumor angiogenesis and cell migration.

Bioapplications of NRP1

Because NRP1 is highly expressed in a variety of tumors and is closely related to tumor growth, invasion and metastasis, it has become an important target for anti-tumor drug development. Currently, a number of antibodies and inhibitors targeting NRP1 have entered clinical trials to evaluate their efficacy and safety in cancer treatment.

High Purity

High Bioactivity

NRP1-2.jpg

Fig2. Activity Data. (NRP1-3346H)

Case study 1: Liang Jin, 2024

Neuropilin-1 (NRP1), a transmembrane glycoprotein, plays an important role in the malignant progression of gliomas; however, its role in chemoresistance is not fully understood. In this study, the researchers observed the effects of NRP1 on the stemness and chemoresistance of glioma cells and the mediating role of Yes-associated protein (YAP). They constructed NRP1 overexpressing LN-229 glioma cells. Cells were treated with recombinant NRP1 protein (rNRP1) and the YAP inhibitor Super-TDU when necessary. MTT assay was used to detect the sensitivity of cells to temozolomide (TMZ). Western blotting was performed to detect cellular CD133, CD44, p-LATS1, and p-YAP protein expression. Immunofluorescence and flow cytometry were used to detect the subcellular localization of YAP and apoptosis, respectively.

The results showed that NRP1 overexpression and rNRP1 treatment enhanced self-renewal, TMZ resistance, and CD133 and CD44 protein expression in LN-229 cells. NRP1 overexpression and rNRP1 treatment also induced LATS1 and YAP dephosphorylation and YAP nuclear translocation. Super-TDU inhibits NRP1 overexpression-induced enhanced self-renewal and TMZ resistance in LN-229 cells.

NRP1-3.jpg

Fig1. LN-229 cells were transfected with rNRP1 treatment for 24 h, and the number and diameter of cell spheres were measured after 7 d of incubation using stem cell sphere culture medium.

NRP1-4.jpg
Fig2. LN-229 cells were treated with rNRP1 (75, 150, 300 ng/mL) for 24 h. Western blotting detected CD133 and CD44 protein expression.

Case study 2: Peng Zhang, 2023

Prostate cancer (PCa) is the most common malignant tumor with a high global incidence in males. The mechanism underlying PCa progression is still not clear. This study observed that NRP1 was highly expressed in PCa and associated with poor prognosis in PCa patients. Functionally, NRP1 depletion attenuated the proliferation and migration ability of PCa cells in vitro and in vivo, while NRP1 overexpression promoted PCa cell proliferation and migration. Moreover, it was observed that NRP1 depletion induced G1 phase arrest in PCa cells. Mechanistically, HIF1α is bound to the specific promoter region of NRP1, thereby regulating its transcriptional activation. Subsequently, NRP1 interacted with EGFR, leading to EGFR phosphorylation. This study also provided evidence that the b1/b2 domain of NRP1 was responsible for the interaction with the extracellular domain of EGFR. Moreover, EGFR mediated NRP1-induced activation of the AKT signaling pathway, which promoted the malignant progression of PCa. In addition, the administration of NRP1 inhibitor EG01377 significantly inactivated the EGFR/AKT signaling axis, thereby suppressing PCa progression.

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Fig3. Immunoblot assay elucidates that ectopic overexpression of NRP1 promotes EGFR phosphorylation level.

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Fig4. Identification of the b1/b2 domain of NRP1 mediating its interaction with EGFR.
NRP1-7.jpg

Fig1. NRP1 regulates multiple signalling pathways in endothelial cells. (Claudio Raimondi, 2016)

NRP1-8.jpg

Fig2. VEGF165 induces complex formation between NRP1 and VEGFR2 to enhance VEGFR2 signaling in endothelial cells in vitro. (Alice Plein, 2014)

NRP1 involved in several pathways and played different roles in them. We selected most pathways NRP1 participated on our site, such as Axon guidance, HTLV-I infection, which may be useful for your reference. Also, other proteins which involved in the same pathway with NRP1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Axon guidanceSCN4A;DCX;SEMA3D;COL9A1;RGS3;EPHA7;CSNK2A1;PLXNA3;EPHA4A
HTLV-I infectionAKT1;CD3G;KAT2A;RAN;CD3E;TRP53;NFATC1;HLA-B;MSX1

NRP1 has several biochemical functions, for example, coreceptor activity, cytokine binding, growth factor binding. Some of the functions are cooperated with other proteins, some of the functions could acted by NRP1 itself. We selected most functions NRP1 had, and list some proteins which have the same functions with NRP1. You can find most of the proteins on our site.

Function Related Protein
coreceptor activityCD28;ZP2;RGMA;CCR8;RAMP3;HFE2;CXCR7B;CCR5;IGSF1
cytokine bindingGBP1;NRP1;ELANE;KIT;TNFRSF11A;CSF1R;KITB;LIFR;NRP2
growth factor bindingLTBP4;NKD2;BMPR2;IGFBP2B;LTBP2;ERBB3;SDCBP;IL6ST;FGFBP2
heparin bindingSERPINE2;SOST;ZNF146;Itgam&Itgb2;ELANE;FMODA;RSPO3;HBEGF;FSTL1
metal ion bindingCYP4A12A;STK32B;TRAF4A;NUDT19;FTR72;TDO2A;CISD3;CYP2C39;PPM1BA
protein bindingTFG;PTPRR;ZBTB9;FAM117B;EIF6;NONO;EXO1;NPM1;ALKBH3
semaphorin receptor activityPLXNA1B;NRP2A;PLXNB2A;PLXND1;PLXNA1;PLXNB2;NRP1B;Plxna2;NRP1
vascular endothelial growth factor bindingPDGFRB;KDR;PDGFRA;NRP1
vascular endothelial growth factor-activated receptor activityFLT3;KDR;NRP2A;FLT4;PDGFRA;NRP1;NRP1A;NRP1B;NRP2B

NRP1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with NRP1 here. Most of them are supplied by our site. Hope this information will be useful for your research of NRP1.

VEGFA; KDR; FLNA; FLT1; RPL27; ptsN1; CSNK2A2

Research Area

Related articles

Weekes, CD; Beeram, M; et al. A phase I study of the human monoclonal anti-NRP1 antibody MNRP1685A in patients with advanced solid tumors. INVESTIGATIONAL NEW DRUGS 32:653-660(2014).
Venkova, K; Christov, A; et al. Semaphorin 3A Signaling Through Neuropilin-1 Is an Early Trigger for Distal Axonopathy in the SOD1(G93A) Mouse Model of Amyotrophic Lateral Sclerosis. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY 73:702-713(2014).
  • Q&As
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Q&As (6)

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What is the role of NRP1 in lung disease? 12/01/2022

It may be involved in the processes of angiography, inflammation, fibrosis and other processes in the lungs, and is related to the occurrence and development of lung diseases.

What factors are associated with high NRP1 expression? 01/02/2022

The high expression of NRP1 may be related to factors such as the aggressiveness of the tumor and the increased angiogenesis of the tumor.

Can NRP1 be targeted for targeted therapy? 03/02/2021

Yes, NRP1, as a molecule with important biological functions and clinical significance, can be targeted as a target therapy.

What are the applications of NRP1 in clinical diagnosis? 10/29/2020

This protein can be used as a potential marker in clinical diagnosis for tumor diagnosis and prognosis evaluation.

Is NRP1 associated with drug therapy? 04/18/2020

Yes, NRP1 may be related to the metabolism of drugs, the response to drug treatment, etc., which affects the efficacy and side effects of drugs.

How is NRP1 related to immune diseases? 04/07/2020

NRP1 may be involved in the regulation of autoimmune responses, inflammatory responses, etc., and is related to the occurrence and development of immune diseases.

Customer Reviews (3)

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Reviews
05/24/2022

    The protein has a moderate molecular weight and is convenient to use.

    07/12/2021

      NRP1 can be easily used and measured in experiment.

      04/20/2021

        NRP1 has a high purity.

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