Acitve Recombinant Human NRP1 protein(Met1-Lys644), His&Avi-tagged, Biotinylated

• Cat.No.: NRP1-1365H
• Product Overview: Biotinylated Recombinant Human NRP1 (NP_001019799.1) (Met1-Lys644) was expressed in HEK293 with a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His&Avi
• Protein length: Met1-Lys644
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized VEGF165 at 10 μg/mL (100 μL/well) can bind Human Neuropilin-1, Biotinylated, the EC50 of Human Neuropilin-1, Biotinylated is 300-600 ng/mL.
• Molecular Mass: The recombinant Human NRP1 consists of 649 amino acids and predicts a molecular mass of 73.1 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NRP1 neuropilin 1 [ Homo sapiens ]
• Official Symbol: NRP1
• Synonyms: NRP1; neuropilin 1; neuropilin-1; CD304; NRP; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; NP1; BDCA4; DKFZp781F1414; DKFZp686A03134
• Gene ID: 8829
• mRNA Refseq: NM_001024628
• Protein Refseq: NP_001019799
• MIM: 602069
• UniProt ID: O14786