Active Recombinant Cynomolgus NRP1 protein

• Cat.No.: NRP1-1219C
• Product Overview: Recombinant Cynomolgus NRP1 protein (G7PEQ1) (Met1-Gly613) was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Non
• Protein Length: Met1-Gly613
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind biotinylated human VEGF165 in functional ELISA.
• Molecular Mass: The recombinant cynomolgus NRP1 is a disulfide-linked homodimer. The reduced monomer comprises 599 amino acids and has a calculated molecular mass of 67.5 KDa.The apparent molecular mass of the protein is approximately 67-77 KDa in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.