Recombinant Human PRMT5 protein, His&FLAG-tagged

• Cat.No.: PRMT5-2321H
• Product Overview: Recombinant Human PRMT5 protein(NP_006128.1) (Ala 2-Leu 637) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus and a Flag tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 2-637 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM EDTA, 0.5PMSF, 0.5mM, TCEP, 25% glycerol.
• Molecular Mass: The recombinant human PRMT5 consists of 655 amino acids and predictes a molecular mass of 75 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPRMT5 is approximately 65 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001034708.1
• Unit ID: O14744
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/O14744

Gene Information

• Gene Name: PRMT5 protein arginine methyltransferase 5 [ Homo sapiens ]
• Official Symbol: PRMT5
• Synonyms: PRMT5; protein arginine methyltransferase 5; HRMT1L5, SKB1, skb1 (S. pombe) homolog, SKB1 homolog (S. pombe); protein arginine N-methyltransferase 5; SKB1Hs; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5; JBP1; SKB1; IBP72; HRMT1L5
• Gene ID: 10419
• mRNA Refseq: NM_001039619
• protein Refseq: NP_001034708
• MIM: 604045