Site-Saturation Mutagenesis

Site-Saturation Mutagenesis Service at Creative BioMart systematically replaces WT amino acid coding sequences with all 19 non–wild type amino acids at the position(s) you specify. Well-known as sequential permutation, site-saturation mutagenesis in combination with a powerful screening assay is the most systematic mutation strategy to identify amino acid substitutions that fulfill your protein engineering goals .

Site-Saturation Mutagenesis Service

Creative BioMart offer several options for sequential permutation to fit a wide array of research needs. You can choose to create variants at one or more positions, and receive constructs individually or pooled. We can carry out:

• Variant constructs are subcloned into the vector of your choice.
• Constructs are sequence-verified .
• Pooled constructs are bulk-sequenced to verify mutation at the requested positions and the nonmutated regions intact.
• Delivery of all 19 non-WT variants at single or multiple site.

• Identify beneficial or detrimental amino acid substitutions.
• Increase affinity, specificity, activity, heat stability, detergent tolerance.
• Change substrate specificity
• Reduce homology between two related proteins (avoid IP issues).
• Identify active sites or receptor-binding sites.

Applications

To conclude, our main advantages:

• Unique control over mutational bias.
• Increased consecutive mutations leading to unexplored protein variants diversity.
• Systematic identification of beneficial amino acid substitutions—check every possible variant at each position.
• Simple and fast library generation.
• Extremely cost-efficient.