IL28RA-5659H
Optional Service: Optional requirements on this protein
Product Overview :
RecombinantHuman IL28RA protein was expressed in Murine myeloma cell line with the Fcpart of Human IgG1 Pro100-Lys330 (C-terminus) and Human IL28RAArg21-Ala228 (N-terminus).
Description :
IL28RA isa type I transmembrane glycoprotein that is the cytokine receptor family 2member 12 (CRF2-12). It pairs with the IL-10 receptor β subunit (IL-10 Rβ,CRF2-4) to form the IL-28 R. Each subunit of this receptor can interact withthe interferon-like cytokines (type III interferons) IL-28A (IFN-λ2), IL-28B(IFN-λ3) or IL-29 (IFN-λ1). Human IL28RA cDNA encodes a 520 amino acid (aa)protein with a 20 aa signal peptide, a 208 aa extracellular domain (ECD) witha fibronectin type III motif and four potential N-glycosylation sites, a 21aa transmembrane sequence, and a proline-rich and acidic 271 aa cytoplasmicdomain.
Source :
Mammalian cells
Form :
Lyophilizedfrom a 0.2 μm filtered solution in PBS.
N-terminalSequence :
Arg21
MolecularWeight :
50.2 kDa (monomer)
Activity :
Measuredby its binding ability in a functional ELISA. In a 100 μL reaction mixturecontaining rhIL28RA/FcChimera at 1 μg/mL and rhIL-28A dilutions at 1-1000 ng/mL, the concentrationof rhIL-28A that produces 50% of the optimal binding response is found to beapproximately 40-200 ng/mL.
Endotoxin Level :
<1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE :
65-75kDa, reducing conditions.
Purity :
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution :
100 μg/mLin sterile PBS
Storage :
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.