| Species : |
Mouse |
| Source : |
Mammalian Cells |
| Tag : |
Fc |
| Protein Length : |
21-227 a.a. |
| Description : |
Il28ra isa type I transmembrane glycoprotein that is cytokine receptor family 2 member12 (CRF2-12). It pairs with the IL-10 receptor β subunit (IL-10 Rβ, CRF2-4)to form the IL-28 R. Each subunit of this receptor can interact with theinterferon-like cytokines (type III interferons) IL-28A (IFN-λ2), IL-28B(IFN-λ3) and IL-29 (IFN-λ1). Mouse IL 28A and IL-28B share 97% amino acid(aa) identity rodents do not express IL-29. Mouse Il28ra cDNA encodes a 535 aaprotein with a 20 aa signal peptide, a 207 aa extracellular domain (ECD) witha fibronectin type III motif and four potential N-glycosylation sites, a 21 aatransmembrane sequence, and a proline-rich and acidic 287 aa cytoplasmicdomain. |
| Form : |
Lyophilizedfrom a 0.2 μm filtered solution in PBS. |
| N-terminalSequence : |
Arg21 |
| MolecularWeight : |
50.6 kDa (monomer) |
| Activity : |
Measuredby its binding ability in a functional ELISA. 1 μg/mL of rm Il28ra FcChimera was mixed with serially diluted rmIL-28B. Following incubation, the Il28ra rmIL-28Bcomplex was captured in a goat anti-mFc-coated plate. Bound IL-28B wasmeasured using biotinylated goat anti-mIL-28B. The concentration of rmIL-28Bthat produces 50% of the optimal binding was found to be approximately 0.7-3.5μg/mL. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
62-70kDa, reducing conditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
200 μg/mLin sterile PBS |
| Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution. |
