Active Recombinant Mouse Il1f6

• Cat.No.: Il1f6-552M
• Product Overview: Recombinant Mouse Il1f6 produced in E. coli is approximately 20.9 kDa, a single non-glycosylated polypeptide chain containing 183 amino acids.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36 beta is reported to be expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy control skin and it can stimulate production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. It beta has two isoforms. IL-36β isoform 2 contains one potential N-linked glycosylation site in its C-terminus, while IL-36β isoform 1 lacks potential N-linked glycosylation sites and four of the conserved β-strands. Within the IL-1 family, IL-36β/IL-1F8 shares 30 %, 32 %, 37 %, 46 %, 34 %, 45 % and 28 % a.a. sequence identity with IL-1 ra, IL-1β, IL-36Ra/IL-1F5, IL-36α/IL-1F6, IL-37/IL-1F7, IL-36γ/IL-1F9 and IL-1F10, respectively.
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.0, 5% trehalose.
• Bio-activity: Fully biologically active when compared to standard. Measured by its binding ability in a functional ELISA with mouse IL-1 Rrp2.
• Molecular Mass: 20.9 kDa
• AA Sequence: MMAFPPQSCV HVLPPKSIQM WEPNHNTMHG SSQSPRNYRV HDSQQMVWVL TGNTLTAVPA SNNVKPVILS LIACRDTEFQ DVKKGNLVFL GIKNRNLCFC CVEMEGKPTL QLKEVDIMNL YKERKAQKAF LFYHGIEGST SVFQSVLYPG WFIATSSIER QTIILTHQRG KLVNTNFYIE SEK
• Endotoxin: Less than 1 EU/μg of rMuIL-36β, 183a.a. as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Il1f6 interleukin 1 family, member 6 [ Mus musculus ]
• Official Symbol: Il1f6
• Synonyms: IL1F6; interleukin 1 family, member 6; interleukin-36 alpha; IL-1F6; Fil1epsilon; FIL1 epsilon; IL-1 epsilon; interleukin-1 epsilon; interleukin-1 homolog 1; interleukin-1 family member 6; interleukin 1 family, member 9; interleukin 1 family, member 6 (epsilon); Fil1; Il1f9; Il36a; IL-1H1; IL1RP2; MGC151479; MGC151481
• Gene ID: 54448
• mRNA Refseq: NM_019450
• Protein Refseq: NP_062323
• Function: cytokine activity; interleukin-1 receptor antagonist activity; interleukin-1 receptor binding