Active Recombinant Human IL36A Protein (Lys6-Phe158), C-His tagged, Animal-free, Carrier-free

• Cat.No.: IL36A-125H
• Product Overview: Recombinant Human IL36A Protein (Lys6-Phe158) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin-36 alpha (IL-36α) is an 18 kDa cytokine with 154 amino acid residues. IL-36α is one of the IL-36 agonists secreted from keratinocytes. When IL-36α binds to the IL-36 receptor, it activates NF-κB and MAPK signaling pathways that induce inflammatory responses. In addition, it also stimulates the production of inflammatory cytokines, such as IFN-γ, TNF-α, and IL-6.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 0.7 ng/mL.
• Molecular Mass: The protein has a calculated MW of 18.05 kDa.
  The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Lys6-Phe158
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: IL36A interleukin 36, alpha [ Homo sapiens (human) ]
• Official Symbol: IL36A
• Synonyms: IL36A; interleukin 36, alpha; IL1F6, interleukin 1 family, member 6 (epsilon); interleukin-36 alpha; FIL1; FIL1E; IL 1F6; IL1(EPSILON); MGC129552; MGC129553; FIL1 epsilon; IL-1 epsilon; interleukin-1 epsilon; IL-1F6 (FIL-1-epsilon); interleukin 1, epsilon; interleukin-1 family member 6; interleukin 1 family, member 6 (epsilon); IL1F6; IL-1F6; FIL1(EPSILON)
• Gene ID: 27179
• mRNA Refseq: NM_014440
• Protein Refseq: NP_055255
• MIM: 605509
• UniProt ID: Q9UHA7