Mouse Anti-Human EGFR Hybridoma

• Cat.No.: CSC-H0002

Specification

• Background: This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.
• Cell Line Description: The antibody is directed against the epidermal growth factor receptor, but does not block the binding of EGF to the receptor.
• Immunogen: Epidermal growth factor (EGF) receptors partially purified from A431 cells
• Immunological Donor: BALB/c mouse spleen
• Fusion Species: Mouse X Mouse Hybridoma
• Myeloma: NS1
• Growth properties: Suspension
• Morphology: Lymphoblast
• Propagation: Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Horse Serum (HS)Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C
• Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C.
• Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
• Reactivity: Anti-epidermal growth factor (EGF) receptor
• Isotype: IgG1
• Target: EGFR
• Storage: Liquid nitrogen
• Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
• Ship: Dry ice

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: Beta urogastrone; EGF; Egf; EGF_HUMAN; Epidermal Growth Factor; HOMG4; OTTHUMP00000219721; OTTHUMP00000219722; Pro epidermal growth factor; URG; Urogastrone
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533
• Chromosome Location: 7p12
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem
• Function: ATP binding; MAP/ERK kinase kinase activity; actin filament binding; double-stranded DNA binding; enzyme binding

Reviews

09/05/2022

The powerful biological activity of EGFR combined with its high purity makes the experimental results highly reproducible, which is of great significance in the field of scientific research.

09/26/2019

Due to the short half-life and high clearance characteristics of EGFR, they can better adapt to the needs of clinical treatment and provide more treatment options for patients.

03/23/2019

The production process of EGFR uses advanced biotechnology, which makes the production process more efficient and environmentally friendly, and reduces the consumption of human and material resources.

Q&As

What biological processes of human embryonic development and tissue regeneration is EGFR associated with?

It is associated with biological processes in human embryonic development and tissue regeneration.

Why does EGFR-inhibiting drug therapy have adverse reactions?

EGFR inhibits adverse drug reactions such as rash, inflammation, and diarrhea during drug treatment.

What is the role of EGFR in tumor treatment?

The role of EGFR in tumor treatment is to act as a molecular marker and can be used as a prognostic indicator and monitor tumor progression; at the same time, EGFR mutations can also be targeted for targeted therapy.

What are the general methods used for EGFR gene mutation testing?

EGFR gene mutation detection generally uses PCR, fluorescence in situ hybridization, and direct sequencing.

What other drugs can EGFR inhibitors be combined with to increase efficacy?

The combination of EGFR inhibitors with chemotherapy drugs, radiotherapy, immunotherapy, etc. can increase the efficacy of treatment and reduce the occurrence of adverse reactions.

How do EGFR mutations affect the efficacy of targeted therapies?

EGFR mutations can have a significant impact on the efficacy of targeted therapies, and patients with mutations have higher response rates.