Expand Expand| Cat.No. : | CSC-H0008 |
| Background : | CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. |
| Cell Line Description : | The antibody is directed against the CD4 molecule of sheep and goats. |
| Depositor : | Dr C Mackay, Basel Institute for Immunology, Basel |
| Immunogen : | Sheep lymphocytes |
| Immunological Donor : | BALB/c mouse spleen |
| Fusion Species : | Mouse X Mouse Hybridoma |
| Myeloma : | Sp2/0 |
| Growth properties : | Suspension |
| Morphology : | Lymphoblast |
| Propagation : | Complete growth medium: DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C |
| Subculturing : | Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. |
| Mycoplasma : | Mycoplasma Status: Negative (MycoAlert Kit) |
| Reactivity : | Anti-CD4, ovine |
| Isotype : | IgG3 |
| Target : | CD4 |
| Storage : | Liquid nitrogen |
| Safety Considerations : | The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship : | Dry ice |
| Gene Name : | CD4 CD4 molecule [ Ovis aries ] |
| Official Symbol : | CD4 |
| Synonyms : | CD 4; CD4 (L3T4); CD4; CD4 antigen (p55); CD4 Antigen; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency; CD4_HUMAN; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; OTTHUMP00000238897; p55; T Cell Antigen T4; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3; T cell surface antigen T4/Leu3; T Cell Surface Antigen T4/Leu3; T Cell Surface Glycoprotein CD4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; W3/25; W3/25 antigen; |
| Gene ID : | 443509 |
| mRNA Refseq : | NM_001129902 |
| Protein Refseq : | NP_001123374 |
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