Recombinant Human CD4 Protein, Fc & Avi-tagged

Cat.No. : CD4-23H
Product Overview : Biotinylated Human CD4, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag. The single lysine residue in the Avitag is enzymatically labeled with biotin.
Description : T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
Source : HEK293
Species : Human
Tag : Fc & Avi
Predicted N Terminal : Lys 26
Molecular Mass : The protein has a calculated MW of 69.7 kDa. As a result of glycosylation, the protein migrates as 80 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE).
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage Buffer : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as rotectant before lyophilization.
SDS-PAGE :

Biotinylated Human CD4, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : CD4
Official Symbol : CD4 CD4 molecule [ Homo sapiens (human) ]
Synonyms : CD4; CD4mut; LEU3; CD4 molecule; T-cell surface glycoprotein CD4; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3
Gene ID : 920
mRNA Refseq : NM_000616
Protein Refseq : NP_000607
MIM : 186940
UniProt ID : P01730

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