Active Recombinant Human Interleukin 16
Cat.No. : | IL16-485H |
Product Overview : | Recombinant H-IL-16 is comprised of 130 amino acids with a molecular weight of 13.5 kDa. |
- Specification
- Gene Information
- Related Products
Cat. No. : | IL16-485H |
Description : | Human IL-16 was originally described as Lymphocyte Chemoattractant Factor (LCF), is produced primarily by CD4(+) and CD8(+) T cells and acts as a chemoattractant for lymphocytes, monocytes, eosinophils, dendritic cells and Langerhans cells. |
Species : | Human |
Source : | E.coli |
Form : | Human IL-16 was lyophilized at 1 mg/ml in 10 mM NaP, 7.5. |
Purity : | Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Bio-activity : | The ED50 as determined by its ability to chemoattract human CD4+ T lymphocytes using a concentration range of 10-100 ng/ml, corresponding to a Specific Activity greater than 1000 IU/mg. |
Physical Appearance : | Sterile Filtered white lyophilized (freeze-dried) powder. |
Solubility : | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg"s/ml, which can be further diluted into other aqueous solutions. |
Purity Greater than 95% as determined by : | Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Protein Content determined by : | UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
Endotoxin Level : | Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
Amino acid sequence : | MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS |
Storage : | Lyophilized Interleukin-16 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL16 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Gene Name : | IL16 interleukin 16 (lymphocyte chemoattractant factor) [ Homo sapiens ] |
Official Symbol : | IL16 |
Synonyms : | IL16; interleukin 16 (lymphocyte chemoattractant factor); LCF; IL-16; prIL-16; FLJ16806; FLJ42735; FLJ44234; HsT19289; OTTHUMP00000190849; OTTHUMP00000190850; lymphocyte chemoattractant factor; HsT19289; Pro-interleukin-16; interleukin 16; lymphocyte chemoattractant factor |
Gene ID : | 3603 |
mRNA Refseq : | NM_004513 |
Protein Refseq : | NP_004504 |
MIM : | 603035 |
UniProt ID : | Q14005 |
Chromosome Location : | 15q26.3 |
Function : | cytokine activity; protein binding |
Products Types
◆ Native Protein | ||
IL16-29736TH | Native Human IL16 | +Inquiry |
◆ Lysates | ||
IL16-5245HCL | Recombinant Human IL16 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionLeukocyte migration assays measure IL16's chemotactic impact on cells.
Flow cytometry and qPCR assess T cell activation markers affected by IL16.
Gene expression arrays reveal IL16's influence on inflammatory gene profiles.
RNA interference used to knock down IL16 expression in cell lines.
Experimental setup for studying IL16's modulation of HIV infection involves viral assays.
Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.
Flow cytometry and confocal microscopy visualize IL16's cellular localization.
Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.
ELISA quantifies secreted IL16 levels from cell culture supernatants.
Strategies like animal models and patient samples explore IL16's role in allergies.
Customer Reviews (3)
Write a reviewSuitable for ELISA.
High specificity in ELISA.
Potent in chemotaxis studies.
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