Active Recombinant Human SRC, GST-tagged

• Cat.No.: SRC-191H

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: Src (p60c-src) is a 60 kDa cytoplasmic tyrosine kinase with functions in the regulation of cell division, cell-matrix adhesion, and cell-cellcell adhesion. Src-GST family tyrosine kinases participate in signal transduction of different classes of membrane receptors, including receptor tyrosine kinases and G-protein coupeled receptors. The SrcGST has been generated by fusing GST N-terminal to human c-Src allowing expression inE. coliand purification by affinity chromatography with GSHbeads. The preparation is >90% homogenous in SDS-PAGE and has a specific activity of >3000 U/mg (1 U is defined as transfer of 1 pMol phosphate to the substrate peptide AAEEEIYGEFEAKKKK per minute at 30°C). The fusion protein has been successfully used to label phosphatase substrates. The GST-Tag facilitates the protein's application in typical GST pull-down assays.
• Form: Liquid. Supplied in 50 mM Tris-HCl pH 8.0, 100 mM NaCl and 1 mM DTT.
• Purity: ≥90% by SDS-PAGE.
• Application: > 650 U/µg (1 U is defined as transfer of 1 pMol phosphate to the substrate peptide AAEEEIYG-EFEAKKKK per minute at 30°C).
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) [ Homo sapiens ]
• Synonyms: SRC; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian); ASV; SRC1; c-SRC; p60-Src; proto-oncogene tyrosine-protein kinase SRC; OTTHUMP00000174476; OTTHUMP00000174477; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma; EC 2.7.10.2; pp60c-src; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogenehomolog
• Gene ID: 6714
• mRNA Refseq: NM_005417
• Protein Refseq: NP_005408
• MIM: 190090
• UniProt ID: P12931
• Chromosome Location: 20q12-q13
• Pathway: Adherens junction; Endocytosis; Epithelial cell signaling in Helicobacter pylori infection; ErbB signaling pathway; Focal adhesion; Gap junction; GnRH signaling pathway; Tight junction; VEGF signaling pathway
• Function: ATP binding; SH2 domain binding; SH3/SH2 adaptor activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; transferase activity