| Cat. No. : |
Il1rn-231M |
| Description : |
Interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring inflammatory inhibitor protein. It inhibits the activity of IL-1α and IL-1β by competitively blocking binding to their type I and type II receptors. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Therapeutically, IL-1Ra may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. |
| Source : |
E. coli. |
| Amino Acid Sequence : |
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Cys-Arg-Pro-Ser-Gly. |
| Physical Appearance : |
White lyophilized (freeze-dried) powder. |
| Purity : |
Greater than 98.0% as determined by: (a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. (b) Gel filtration chromatography under non-denaturing conditions. |
| Formulation : |
Interleukin-1Ra is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3. |
| Solubility : |
It is recommended to reconstitute the lyophilized IL-1Ra in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions containing carrier protein such as BSA, HSA or similar. |
| Endotoxin : |
Less than 0.1 ng/µg (IEU/µg) of oplGH (22 K). |
| Protein Content : |
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences. |
| Dimers And Aggregates : |
Less than 2% as determined by gel filtration under non-denaturing conditions. |
| Biological Activity : |
IL-1Ra is capable of inhibitinf IL-1-alpha activity in helper T cell line D10.G4.1 Endotoxin: Less than 0.05 ng/µg (0.5 EU/µg) of recombinant IL-1α. |
| Stability : |
Lyophilized IL-1Ra although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution Interleukin-3 should be stored at 4 ℃ between 2-7 days and for future use below -18 ℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
