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Recombinant Mouse Interleukin-1 Receptor Antagonist

Cat. No. : Il1rn-231M
Product Overview : Recombinant mouse IL-1Ra produced inE. coliis a 17.4 kDa protein consisting of 153 amino acid residues. IL-1Ra is purified by proprietary chromatographic techniques.
Description : Interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring inflammatory inhibitor protein. It inhibits the activity of IL-1α and IL-1β by competitively blocking binding to their type I and type II receptors. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Therapeutically, IL-1Ra may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease.
Source : E. coli.
Amino Acid Sequence : The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Cys-Arg-Pro-Ser-Gly.
Physical Appearance : White lyophilized (freeze-dried) powder.
Purity : Greater than 98.0% as determined by: (a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. (b) Gel filtration chromatography under non-denaturing conditions.
Formulation : Interleukin-1Ra is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3.
Solubility : It is recommended to reconstitute the lyophilized IL-1Ra in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions containing carrier protein such as BSA, HSA or similar.
Endotoxin : Less than 0.1 ng/µg (IEU/µg) of oplGH (22 K).
Protein Content : Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences.
Dimers And Aggregates : Less than 2% as determined by gel filtration under non-denaturing conditions.
Biological Activity : IL-1Ra is capable of inhibitinf IL-1-alpha activity in helper T cell line D10.G4.1 Endotoxin: Less than 0.05 ng/µg (0.5 EU/µg) of recombinant IL-1α.
Stability : Lyophilized IL-1Ra although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution Interleukin-3 should be stored at 4 ℃ between 2-7 days and for future use below -18 ℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Gene Name : Il1rn interleukin 1 receptor antagonist [ Mus musculus ]
Synonyms : interleukin 1 receptor antagonist; IL-1ra; F630041P17Rik; Il1rn; OTTMUSP00000012801; OTTMUSP00000012802; interleukin-1 receptor antagonist protein; IRAP; IL-1RN; IL1 inhibitor
Gene ID : 16181
mRNA Refseq : NM_001039701
Protein Refseq : NP_001034790
UniProt ID : P25085
Chromosome Location : 2 A3; 2 10.0 cM
Function : interleukin-1 receptor antagonist activity; receptor activity

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