Cat. No. : |
FLT3-545H |
Product Overview : |
Recombinant human FLT3 was expressed by baculovirus inSf9 cells usingan N-terminal His tag. MW=54.2kDa. |
Description : |
FLT3 is a receptor tyrosine kinase that has been shown to play a role in proliferation and survival of hematopoietic progenitor cells as well as differentiation of early B lymphoid progenitors. FLT3 consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. FLT3 is the most frequently mutated gene in cases of acute myelogenous leukemia (AML). About 30 to 35% of patients have either internal tandem duplications (ITDs) in the juxtamembrane domain or mutations in the activating loop of FLT3. The consequence of either FLT3-ITD or activating loop mutations is the constitutive activation of the tyrosine kinase activity. |
Source : |
Sf9 insect cells using baculovirus. |
Purity : |
>80%. |
Specific Activity : |
18 pmol/min/μg. Assay condition: The enzyme reaction was carried out for 1h at room temperature in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 μM of ATP. Substrate : 2 μM Tyr peptide 2 from Invitrogen. |
Application : |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Formulated In : |
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT. |
Stability : |
>6 months at –80°C. |