Recombinant Cynomolgus FLT3 protein, hFc-tagged

• Cat.No.: FLT3-1507C
• Product Overview: Recombinant Cynomolgus FLT3 protein(A0A2K5VQ48)(Gln28-Ser541), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: C-hFc
• Protein Length: Gln28-Ser541
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human FLT3 Ligand, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus FLT3, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 84.58 kDa. Due to glycosylation, the protein migrates to 160-180 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.