Recombinant Human Interleukin 32

• Cat.No.: IL32-301H
• Product Overview: Recombinant Human IL-32 alpha produced in E.coli is a 131 amino acid non-glycosylated polypeptide with a molecular mass of 14.9 kDa.MW = 14.9 kDa.

Specification

• Cat. No.: IL32-301H
• Description: Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-α, and MIP-2 production from human & mouse macrophage cell lines. It is up-regulated in activated T- & NKcells, and IFN-γ-treated epithelial cells.
• Source: E.coli.
• Purity: Greater than 97% (determined by SDS-PAGE).
• Form: Lyophilized from a protein solution containing 50 mM NaP, pH 7.5.
• Endotoxin Level: < 0.1 ng per μg of rHu IL-32 alpha (1EU/μg).
• Specific Activity: NA.
• Biological Activity: Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED₅₀for this assay is in the range of 0.125-1.0 μg/ml.
• Reconstitution: Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
• Storage & Stability: The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-32 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: IL32 interleukin 32 [ Homo sapiens ]
• Synonyms: IL32; interleukin 32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma; natural killer cells protein 4; natural killer cell transcript 4; tumor necrosis factor alpha-inducing factor; Interleukin-32; IL-32
• Gene ID: 9235
• mRNA Refseq: NM_001012631
• Protein Refseq: NP_001012649
• MIM: 606001
• UniProt ID: P24001
• Chromosome Location: 16p13.3
• Function: cytokine activity