- Human(21)
Recombinant Human Interleukin 32
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Cat.No. : | IL32-301H Optional Service: Optional requirements on this protein |
Product Overview : | Recombinant Human IL-32 alpha produced in E.coli is a 131 amino acid non-glycosylated polypeptide with a molecular mass of 14.9 kDa.MW = 14.9 kDa. |
Description : | Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-α, and MIP-2 production from human & mouse macrophage cell lines. It is up-regulated in activated T- & NKcells, and IFN-γ-treated epithelial cells. |
Source : | E.coli. |
Purity : | Greater than 97% (determined by SDS-PAGE). |
Form : | Lyophilized from a protein solution containing 50 mM NaP, pH 7.5. |
Endotoxin Level : | < 0.1 ng per μg of rHu IL-32 alpha (1EU/μg). |
Specific Activity : | NA. |
Biological Activity : | Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50for this assay is in the range of 0.125-1.0 μg/ml. |
Reconstitution : | Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. |
Storage & Stability : | The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-32 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles. |
Gene Name : | IL32 interleukin 32 [ Homo sapiens ] |
Synonyms : | IL32; interleukin 32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma; natural killer cells protein 4; natural killer cell transcript 4; tumor necrosis factor alpha-inducing factor; Interleukin-32; IL-32 |
Gene ID : | 9235 |
mRNA Refseq : | NM_001012631 |
Protein Refseq : | NP_001012649 |
MIM : | 606001 |
UniProt ID : | P24001 |
Chromosome Location : | 16p13.3 |
Function : | cytokine activity |
◆ Recombinant Protein
IL32-190H | Recombinant Active Human IL32 Protein, His-tagged(C-ter) | +Inquiry |
IL32-00H | Recombinant Human IL32 Protein, His-tagged | +Inquiry |
IL32-5189H | Recombinant Human IL32 Protein, GST-tagged | +Inquiry |
IL32-173H | Recombinant Human IL-32α Protein | +Inquiry |
IL32-022H | Recombinant Human IL-32 Protein, His-tagged | +Inquiry |
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◆ Lysates
IL32-2743HCL | Recombinant Human IL32 cell lysate | +Inquiry |
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IL10IL10RAIL10RBIL11IL11RAIl11ra1IL12IL12AIL12BIL12RB1IL13IL13RA1IL13RA2IL15IL15RAIL16IL17AIL17BIl17cIL17DIl17fIL17RAIL17RDIL18IL18BPIL18R1IL18RAPIl19Il1aIl1bIL1F10IL1R1Il1r2IL1RAPIL1RAPL1IL1RAPL2IL1RL1IL1RL2Il1rnIL2Il20IL20RAIL21IL21RIL22IL22BPIL22RA1IL22RA2IL23AIL24Il25IL27IL27RAIL28AIl28bIL29IL2RAIL2RBIl2rgIL3IL31IL32Il33Il34IL3RAIL4IL4RIl4raIl5IL5RAIl6IL6RIl6raIL6STIl7IL7RIL8IL9IL36AIl12rb2IL17RBIL23RIL26IL31RAIL36BIL36GIL36RNIL37IL9RIL17RCIL17REIL28RAIL4I1IL20RBIL17RELIl11ra2IL27bIL7RAIl23a&Il12b
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