Species : |
Human |
Source : |
E.coli |
Tag : |
Non |
Description : |
Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-α, and MIP-2 production from human & mouse macrophage cell lines. It is up-regulated in activated T- & NKcells, and IFN-γ-treated epithelial cells. |
Purity : |
Greater than 97% (determined by SDS-PAGE). |
Form : |
Lyophilized from a protein solution containing 50 mM NaP, pH 7.5. |
Endotoxin Level : |
< 0.1 ng per μg of rHu IL-32 alpha (1EU/μg). |
Specific Activity : |
NA. |
Biological Activity : |
Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50for this assay is in the range of 0.125-1.0 μg/ml. |
Reconstitution : |
Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. |
Storage & Stability : |
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-32 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles. |