Recombinant Human Interleukin 32 IL32-301H

Recombinant Human Interleukin 32


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Recombinant Human Interleukin 32

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Cat.No. : IL32-301H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human IL-32 alpha produced in E.coli is a 131 amino acid non-glycosylated polypeptide with a molecular mass of 14.9 kDa.MW = 14.9 kDa.
Description : Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-α, and MIP-2 production from human & mouse macrophage cell lines. It is up-regulated in activated T- & NKcells, and IFN-γ-treated epithelial cells.
Source : E.coli.
Purity : Greater than 97% (determined by SDS-PAGE).
Form : Lyophilized from a protein solution containing 50 mM NaP, pH 7.5.
Endotoxin Level : < 0.1 ng per μg of rHu IL-32 alpha (1EU/μg).
Specific Activity : NA.
Biological Activity : Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50for this assay is in the range of 0.125-1.0 μg/ml.
Reconstitution : Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability : The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-32 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Gene Name : IL32 interleukin 32 [ Homo sapiens ]
Synonyms : IL32; interleukin 32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma; natural killer cells protein 4; natural killer cell transcript 4; tumor necrosis factor alpha-inducing factor; Interleukin-32; IL-32
Gene ID : 9235
mRNA Refseq : NM_001012631
Protein Refseq : NP_001012649
MIM : 606001
UniProt ID : P24001
Chromosome Location : 16p13.3
Function : cytokine activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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