| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
Tumor necrosis factor α (TNF-α;), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α; occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α; is glycosylated, but non-glycosylated recombinant TNF-α; has comparable biological activity. The biologically active native form of TNF-α; is reportedly a trimer. Human and murine TNF-α; show approximately 79% homology at the amino acid level and crossreactivity between the two species. Two types of receptors for TNF-α; have been described and virtually all cell types studied show the presence of one or both of these receptor types. |
| Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
| Bio-activity : |
The ED50 determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of >2.0 × 107 IU/mg in the presence of actinomycin D. |
| Molecular Mass : |
Approximately 18.3 kDa, a single, non-glycosylated polypeptide chain containing 157 amino acids with 6 × His at N-terminus. |
| AA Sequence : |
MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL |
| Endotoxin : |
Less than 1 EU/μg of TNF-α;, His as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Usage : |
This material is offered by Creatvie Biomart for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE. |
| Storage Buffer : |
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at< -20°c="" further="" dilutions="" should="" be="" made="" in="" appropriate="" buffered=""> |
