Tag : |
Non |
Size : |
96 reactions |
Description : |
The Fluorogenic HDAC 8 Assay Kit is a complete assay system designed to measure histone deacetylase (HDAC) 8 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC8 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC 8 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only twoa simple steps on a microtiter plate are needed to analyze the HDAC 8 activity level. First, the HDAC fluorometric substrate is incubated with purified HDAC8 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader. |
Applications : |
Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC8 for drug discovery and HTS applications. |
Storage : |
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
Kit Components : |
HDAC8 human recombinant enzyme: 2 µg; -80°C Fluorogenic HDAC substrate class 2A (5 mM): 50 µl; -80°C 2x HDAC Developer (contains Trichostatin A) (50 µM): 6 ml; -80°C Trichostatin A (1 mM) in DMSO: 100 µl; -20°C HDAC Assay Buffer: 10 ml; -20°C black, low binding NUNC black microtiter plate: 1 plate; Room temp. |