PDE4A Assay Kit

• Cat.No.: Kit-1838
• Product Overview: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4A is widely expressed in brain tumors and promotes their growth and its inhibitor Rolipram has been identified as a potent anti-inflammatory agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.

Specification

• Size: 96 reactions
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Storage: At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Reviews

12/05/2022

PDE4A requires less storage environment.

07/26/2022

The expression of PDE4A was stable.

02/08/2022

It has the advantage of easy operation in the expression system and can improve the expression efficiency.

Q&As

How effective are PDE4A inhibitors in the treatment of asthma?

The inhibitors have been used in the treatment of asthma, and studies have shown that they can significantly reduce the number of asthma attacks and the use of drug D.

How to introduce the structure of PDE4A?

This protein has multiple conserved regions and different subtypes, which is of great significance for the selective inhibition of drugs.

What is a selective PDE4A inhibitor?

Selective PDE4A inhibitors refer to drug molecules that target only the PDE4A protein and do not inhibit other PDE4 homologs.

What is the mechanism by which PDE4A regulates T cell-mediated autoimmune responses?

It plays an important role in the activation and regulation of T cells by regulating cAMP levels, and is involved in T cell-mediated autoimmune responses.

Are levels of PDE4A associated with sleep disturbances?

Studies have shown that PDE4A depletion may be related to sleep-wake behavior and time stress response, but its specific role needs to be further studied.

What is the expression pattern of PDE4A in T cells?

In T cells, PDE4A is mainly expressed in the cytoplasm and peripheral regions of the nuclear membrane.