Recombinant Human CXCL13 293 Cell Lysate

• Cat.No.: CXCL13-7170HCL

Specification

• Description: Antigen standard for chemokine (C-X-C motif) ligand 13 (CXCL13) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: CXCL13 chemokine (C-X-C motif) ligand 13 [ Homo sapiens ]
• Official Symbol: CXCL13
• Synonyms: CXCL13; chemokine (C-X-C motif) ligand 13; SCYB13, small inducible cytokine B subfamily (Cys X Cys motif), member 13 (B cell chemoattractant); C-X-C motif chemokine 13; ANGIE; ANGIE2; B cell chemoattractant; BCA 1; BLC; BLR1L; CXC chemokine BLC; B-cell chemoattractant; B-lymphocyte chemoattractant; b lymphocyte chemoattractant; small-inducible cytokine B13; B-cell-attracting chemokine 1; b cell-attracting chemokine 1; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); B-cell-homing chemokine (ligand for Burkitts lymphoma receptor-1); small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); BCA1; BCA-1; SCYB13
• Gene ID: 10563
• mRNA Refseq: NM_006419
• Protein Refseq: NP_006410
• MIM: 605149
• UniProt ID: O43927
• Chromosome Location: 4q21
• Pathway: CXCR3-mediated signaling events, organism-specific biosystem; Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem
• Function: CCR10 chemokine receptor binding; CXCR3 chemokine receptor binding; CXCR5 chemokine receptor binding; chemokine activity; fibroblast growth factor binding; heparin binding; protein heterodimerization activity; receptor agonist activity

Reviews

07/10/2021

Stability of CXCL13 was very good during long-term storage, and its properties remained unchanged after prolonged storage, indicating its excellent storage stability.

06/21/2021

The broad storage conditions of proteins make the experimental process more stable and reliable, which provides strong support for scientific research.

08/14/2019

In the process of CXCL13 production, it can be seen that it pays great attention to purity and biological activity, which makes its product extremely reliable.

Q&As

Is the expression pattern of CXCL13 protein related to the clinical prognosis of specific diseases?

Expression pattern of CXCL13 protein may be related to the clinical prognosis of specific diseases, but the specific association needs further research and verification.

What are the expression characteristics of CXCL13 protein in immune cells?

The protein is mainly expressed in immune cells by activated dendritic cells, macrophages and inflammatory cells.

Is there a regulatory mechanism for CXCL13 protein expression during inflammation?

The expression of CXCL13 protein is regulated by a variety of signaling pathways, including NF-κB, AP-1, etc. Stimulation of inflammatory factors and cytokines may lead to upregulation of CXCL13 expression.

What is the role of CXCL13 protein in lymph node metastasis?

In lymph node metastasis, the CXCL13 protein promotes the localization and metastasis of cancer cells by attracting CXCR5-positive cancer cells or immune cells.

What are the implications of CXCL13 protein research for new drug development?

CXCL13 protein plays an important role in immune regulation and inflammatory response, and studying the function and regulatory mechanism of CXCL13 protein will help the development of new drugs, such as targeted drugs targeting CXCL13 and its related signaling pathways, which may be applied to the treatment of related diseases.

What are the potential applications of CXCL13 protein in immunotherapy?

The CXCL13 protein is involved in immunomodulation, and its bound antibodies or specific receptor antagonists may contribute to the design and development of immunotherapy.