Recombinant Human FLT3 protein, His & T7-tagged

• Cat.No.: FLT3-763H
• Product Overview: Recombinant Human FLT3 aa. (Lys334~Ser543 (Accession # P36888)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.9kDa
• Protein length: Lys334~Ser543 (Accession # P36888)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 8.3.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: FLT3 fms related tyrosine kinase 3 [ Homo sapiens (human) ]
• Official Symbol: FLT3
• Synonyms: FLT3; fms related tyrosine kinase 3; FLK2; STK1; CD135; FLK-2; receptor-type tyrosine-protein kinase FLT3; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; growth factor receptor tyrosine kinase type III; stem cell tyrosine kinase 1
• Gene ID: 2322
• mRNA Refseq: NM_004119.2
• Protein Refseq: NP_004110.2
• UniProt ID: P36888