| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
hFc |
| Form : |
Lyophilized from sterile PBS , pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
| Bio-activity : |
1. Measured by its binding ability in a functional ELISA. Immobilized human IL17BR-His at 10 μg/ml (100 μl/well) can bind human Fc-IL25, The EC50 of human Fc-IL25 is 1.7-4.2 μg/mL.2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 15-60 ng/mL. |
| Molecular Mass : |
The recombinant human IL25/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and predicts a molecular mass of 45 KDa. As a result of glycosylation, the apparent molecular mass of rh IL25/Fc monomer is approximately 50-60 KDa in SDS-PAGE under reducing conditions. |
| Proteinlength : |
Tyr33-Gly177 |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
