Recombinant Human IL25 cell lysate

Cat.No. : IL25-2920HCL
Product Overview : Human IL25 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL25 interleukin 25 [ Homo sapiens ]
Official Symbol : IL25
Synonyms : IL25; interleukin 25; IL17E, interleukin 17E; interleukin-25; IL 17E; IL 25; interleukin 17E; interleukin-17E; IL17E;
Gene ID : 64806
mRNA Refseq : NM_022789
Protein Refseq : NP_073626
MIM : 605658
UniProt ID : Q9H293
Chromosome Location : 14q11.2
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
Function : cytokine activity; interleukin-17E receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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01/29/2021

    The activity is very strong and can meet my research needs.

    08/31/2019

      Price is very reasonable and good value for money.

      01/05/2019

        It is very stable and can be stored and used for a long time.

        Q&As (6)

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        Is there any research showing that IL-25 is effective in tumor treatment? 12/10/2022

        There is a lack of conclusive evidence that IL-25 has a clear effect in tumor treatment, but there are some studies underway.

        Are there any side effects to IL-25? 09/22/2021

        There are no clear studies on the side effects of IL-25, but the patient response needs to be closely monitored during research and clinical application.

        Has the study found potential applications of IL-25 in the treatment of other diseases? 01/18/2021

        In addition to treating asthma and inflammatory bowel disease, IL-25 may also have potential applications in the treatment of lung infections, urticaria and other diseases.

        What is the mechanism of action of IL-25? 12/18/2020

        IL-25 activates specific signaling pathways by binding to specific receptors, thereby regulating the activity of immune cells and the degree of inflammation in the body.

        Is there any research showing that IL-25 is effective against certain diseases? 08/17/2020

        Some studies have shown that recombinant IL-25 can improve symptoms of diseases such as asthma and inflammatory bowel disease, but more research is needed to verify its efficacy.

        Does IL-25 therapy need to be administered by injection? 03/28/2019

        Yes, in general, IL-25 therapy needs to be administered by injection to ensure that the protein can enter the body and work.

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