| Species : |
Rat |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
Rat IL-17E (IL-17), is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17E protein is a homodimer consisting of two 145 amino acids peptides. |
| Form : |
Lyophilized with no additives. |
| Purity : |
Purity Greater than 97% as determined by: Reducing and non-reducing SDS-PAGE. |
| Bio-activity : |
Rat IL-17E activity is measured by the dose-dependent induction of mG-CSF production in cultured NIH 3T3 cells. The dose range for this activity is 10-100ng/ml. |
| Physical Appearance : |
Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility : |
It is recommended to reconstitute the lyophilized product with sterile 20 mM HCl at a concentration of 0.1 mgμs/ml. Further dilutions into other buffers can be made. |
| Purity Greater than 97% as determined by : |
Reducing and non-reducing SDS-PAGE. |
| Protein Content determined by : |
UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level : |
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence : |
CSHLPRCCPS KQQEFPEEWL KWNPAPVSPP EPLRHTHHPE SCRASKDGPL NSRAISPWSY ELDRDLNRVP QDLYHARCLC PHCVSLQTGS HMDPMGNSVP LYHNQTVFYR RPCHGEQGAH GRYCLERRLY RVSLACVCVR PRMMA |
| Storage : |
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
