Recombinant Rat Interleukin 25

• Cat.No.: Il25-90R
• Product Overview: Recombinant Rat Interleukin-17E produced inE.Coliis a homodimeric, non-glycosylated polypeptide chain containing 145 amino acids and having a molecular mass of 35.5 kDa. The Rat IL-25 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: Il25-90R
• Description: IL-25 also called IL-17E cytokine has a sequence similarity with IL17. IL-17E indluces NF-kappaB activation, and stimulates the production of IL-8. IL17E and IL17B are ligands for the cytokine receptor IL17BR. IL-25 is a proinflammatory cytokine favoring Th2-type immune response. The upregulation of costimulation-induced IL-17E receptors and release of cytokines and chemokines from IL-17E treated costimulated Th cells are differentially regulated by intracellular JNK, p38 MAPK and NF-kappaB activity. Blocking Iinterleukin-25 prevents airway hyperresponsiveness, a critical feature of clinical asthma. IL25 produced by innate effector eosinophils and basophils increase the allergic inflammation by enhancing the maintenance and functions of TSLP-DC activated adaptive Th2 memory cells. Over expression of IL-25 up-regulates gene expression of Th2 cytokines and induces growth retardation, jaundice, and multiorgan inflammation in a transgenic mouse model. IL-25 contributes to the induction and maintenance of eosinophilic inflammation by acting on lung fibroblasts which supports the fact that IL-17E is an important factor in asthma pathophysiology. IL-17E operates by amplifying TH2 cell-mediated allergic airway inflammation but doesn't induce allergic inflammation in vivo.
• Source: Escherichia Coli.
• Amino Acid Sequence: CSHLPRCCPS KQQEFPEEWL KWNPAPVSPP EPLRHTHHPE SCRASKDGPL NSRAISPWSY ELDRDLNRVP QDLYHARCLC PHCVSLQTGS HMDPMGNSVP LYHNQTVFYR RPCHGEQGAH GRYCLERRLY RVSLACVCVR PRMMA
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized from a concentrated (1mg/ml) solution containing no additives.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin Rat IL25 in sterile 10 mM HCL not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: Rat IL-17A is fully biologically active when compared to standards via dose-dependent induction of IL-6 production in cultured rat fibroblasts.
• Stability: Lyophilized Interleukin Rat IL17E although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat IL-25 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Il25 interleukin 25 [ Rattus norvegicus ]
• Synonyms: Il25; interleukin 25; RGD1561632;rgd1561632_predicted; similar to IL25; similar to il25 (predicted); similar to IL25 (predicted); RGD1561632_predicted; LOC501996
• Gene ID: 501996
• mRNA Refseq: XM_577423
• Protein Refseq: XP_577423
• Chromosome Location: 15p13
• Pathway: Cytokine-cytokine receptor interaction
• Function: cytokine activity

Reviews

01/29/2021

The activity is very strong and can meet my research needs.

08/31/2019

Price is very reasonable and good value for money.

01/05/2019

It is very stable and can be stored and used for a long time.

Q&As

Is there any research showing that IL-25 is effective in tumor treatment?

There is a lack of conclusive evidence that IL-25 has a clear effect in tumor treatment, but there are some studies underway.

Are there any side effects to IL-25?

There are no clear studies on the side effects of IL-25, but the patient response needs to be closely monitored during research and clinical application.

Has the study found potential applications of IL-25 in the treatment of other diseases?

In addition to treating asthma and inflammatory bowel disease, IL-25 may also have potential applications in the treatment of lung infections, urticaria and other diseases.

What is the mechanism of action of IL-25?

IL-25 activates specific signaling pathways by binding to specific receptors, thereby regulating the activity of immune cells and the degree of inflammation in the body.

Is there any research showing that IL-25 is effective against certain diseases?

Some studies have shown that recombinant IL-25 can improve symptoms of diseases such as asthma and inflammatory bowel disease, but more research is needed to verify its efficacy.

Does IL-25 therapy need to be administered by injection?

Yes, in general, IL-25 therapy needs to be administered by injection to ensure that the protein can enter the body and work.