Recombinant Mouse IL25 cell lysate
|Product Overview :||Mouse IL25 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls|
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|Source :||Human cells|
|Preparation method :||Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.|
|Lysis buffer :||Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Quality control Testing :||12.5% SDS-PAGE Stained with Coomassie Blue|
|Recommended Usage :||1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.|
|Stability :||Samples are stable for up to twelve months from date of receipt at -80°C|
|Storage Buffer :||50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Storage Instruction :||Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.|
|Gene Name :||Il25 interleukin 25 [ Mus musculus ]|
|Official Symbol :||IL25|
|Synonyms :||IL25; interleukin 25; interleukin-25; interleukin 17E; Il17e; IL-17e;|
|Gene ID :||140806|
|mRNA Refseq :||NM_080729|
|Protein Refseq :||NP_542767|
|Pathway :||Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;|
|Function :||cytokine activity; interleukin-17E receptor binding;|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (6)Ask a question
There is a lack of conclusive evidence that IL-25 has a clear effect in tumor treatment, but there are some studies underway.
There are no clear studies on the side effects of IL-25, but the patient response needs to be closely monitored during research and clinical application.
In addition to treating asthma and inflammatory bowel disease, IL-25 may also have potential applications in the treatment of lung infections, urticaria and other diseases.
IL-25 activates specific signaling pathways by binding to specific receptors, thereby regulating the activity of immune cells and the degree of inflammation in the body.
Some studies have shown that recombinant IL-25 can improve symptoms of diseases such as asthma and inflammatory bowel disease, but more research is needed to verify its efficacy.
Yes, in general, IL-25 therapy needs to be administered by injection to ensure that the protein can enter the body and work.
Customer Reviews (3)Write a review
Efficacy of the protein can be evaluated by observing its pharmacokinetics, pharmacodynamics and toxicity in mice or rats. -
Animal models were used to evaluate the efficacy and side effects of the remade proteins. -
Storage is stable, and the environmental requirements are not harsh. -
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