Active Recombinant Mouse Il25 protein, His-tagged, Low Endotoxin

• Cat.No.: IL25-2161M
• Product Overview: Recombinant Mouse Il25 protein(Q8VHH8)(17-169 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 17-169 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse IL-25, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti IL-25 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 19.08 kDa. Due to glycosylation, the protein migrates to 27-35 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Il25 interleukin 25 [ Mus musculus ]
• Official Symbol: Il25
• Synonyms: IL25; interleukin 25; interleukin-25; interleukin 17E; Il17e; IL-17e
• Gene ID: 140806
• mRNA Refseq: NM_080729
• Protein Refseq: NP_542767