MAP3K14-894H
Optional Service: Optional requirements on this protein
Product Overview :
Human NIK, full length, amino acids M1-P947 (as in NCBI/Protein entry NP_003945.2), N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells.
Source :
Insect cell
Species :
Human
Tag :
His/GST
Bio-activity :
Specific kinase activity (Pi transfer): 1 pmol/µg × min ATP-KM: 17 µM
Molecular Mass :
133.437 kDa
Storage :
At -80 centigrade. For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10µl are not advisable. Avoid repeated freeze-thaw cycles!
Concentration :
0.131 µg/µl
Storage Buffer :
50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20 % glycerol
Coomassie stain :
Determination of Vmax and KM value for ATP :
Determination of KM value / Specific activity: • Assay conditions: 60 mM HEPES-NaOH, pH 7.5 3 mM MgCl2 3 mM MnCl2 3 µM Na-orthovanadate 1.2 mM DTT 50 µg/ml PEG20.000 ATP (variable) Substrate: CDC25C-derived peptide, 20 µg/ml Kinase: 2 µg/ml • Filter binding assay MSPH membrane (Millipore)
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.