Recombinant Human MAP3K14 Protein, His/GST-tagged MAP3K14-894H

Recombinant Human MAP3K14 Protein, His/GST-tagged

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Recombinant Human MAP3K14 Protein, His/GST-tagged

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Cat.No. : MAP3K14-894H Optional Service: Optional requirements on this protein
Product Overview : Human NIK, full length, amino acids M1-P947 (as in NCBI/Protein entry NP_003945.2), N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells.
Source : Insect cell
Species : Human
Tag : His/GST
Bio-activity : Specific kinase activity (Pi transfer): 1 pmol/µg × min
ATP-KM: 17 µM
Molecular Mass : 133.437 kDa
Storage : At -80 centigrade.
For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10µl are not advisable. Avoid repeated freeze-thaw cycles!
Concentration : 0.131 µg/µl
Storage Buffer : 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20 % glycerol
Coomassie stain :

Determination of Vmax and KM value for ATP :

Determination of KM value / Specific activity:
• Assay conditions:
60 mM HEPES-NaOH, pH 7.5
3 mM MgCl2
3 mM MnCl2
3 µM Na-orthovanadate
1.2 mM DTT
50 µg/ml PEG20.000
ATP (variable)
Substrate: CDC25C-derived peptide, 20 µg/ml
Kinase: 2 µg/ml
• Filter binding assay
MSPH membrane (Millipore)

Gene Name : MAP3K14 mitogen-activated protein kinase kinase kinase 14 [ Homo sapiens (human) ]
Official Symbol : MAP3K14
Synonyms : MAP3K14; mitogen-activated protein kinase kinase kinase 14; HS; NIK; HSNIK; FTDCR1B; mitogen-activated protein kinase kinase kinase 14; NF-kappa-beta-inducing kinase; serine/threonine-protein kinase NIK; EC 2.7.11.25
Gene ID : 9020
mRNA Refseq : NM_003954
Protein Refseq : NP_003945
MIM : 604655
UniProt ID : Q99558
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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