Recombinant Mouse IL12A & IL12B Overexpression Lysate(Met 1-Ala 215&Met 1-Ser 335)

Cat.No. : IL12A & IL12B-1782MCL
Product Overview : Recombinant Mouse IL12A (P43431) (Met 1-Ala 215) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant Mouse IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified in HEK293.
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Source : HEK293
Species : Mouse
Tag : C-His-hFc
Protein length : Met 1-Ala 215&Met 1-Ser 335
Form : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Molecular Mass : The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12A is Fc chimera, comprises 758 (434 +324) amino acids and has a calculated molecular mass of 85.9 (48.7 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 45 kDa respectively in SDS-PAGE under reducing conditions.
Storage : Store at 4°C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80°C for up to twelve months. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Il12a interleukin 12a [ Mus musculus ]
Official Symbol : IL12A
Synonyms : IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232;
Gene ID : 16159
mRNA Refseq : NM_001159424
Protein Refseq : NP_001152896

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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