Recombinant Cynomolgus IL12A & IL12B protein, His-tagged

• Cat.No.: IL12A & IL12B-1403C
• Product Overview: Recombinant Cynomolgus IL12A & IL12B protein(G7P6S2(IL12B)&XP_005546300.2(IL12A))(Ile23-Ser328(IL12B) & Arg57-Ser253(IL12A)), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: C-His
• Protein Length: Ile23-Ser328(IL12B) & Arg57-Ser253(IL12A)
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Cynomolgus IL-12, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 34.76 kDa (IL-12B) and 23.64 kDa (IL-12A). Due to glycosylation, the protein migrates to 45-48 kDa (IL-12B) and 38-44 kDa (IL-12A) based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.