Recombinant Mouse IL12A & IL12B protein, His-tagged

• Cat.No.: IL12A & IL12B-1580M
• Product Overview: Recombinant Mouse IL12A & IL12B protein(P43431(IL12A)&P43432(IL12B))(Arg23-Ala215(IL12A) & Met23-Ser335(IL12B)), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: C-His
• Protein Length: Arg23-Ala215(IL12A) & Met23-Ser335(IL12B)
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.5 - 2 ng/mL.
• Molecular Mass: The protein has a predicted MW of 22.80 kDa(IL-12A)&35.79 kDa(IL-12B). Due to glycosylation, the protein migrates to 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Il12a interleukin 12a [ Mus musculus ]
• Official Symbol: IL12A
• Synonyms: IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232
• Gene ID: 16159
• mRNA Refseq: NM_001159424
• Protein Refseq: NP_001152896
• Gene Name 2: Il12b interleukin 12b [ Mus musculus ]
• Official Symbol 2: IL12B
• Synonyms 2: IL12B; interleukin 12b; interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; p40; Il-12b; Il12p40; Il-12p40
• Gene ID 2: 16160
• mRNA Refseq 2: NM_008352
• Protein Refseq 2: NP_032378