Active Recombinant Mouse IL12A & IL12B Heterodimer Protein, Fc-tagged
Cat.No. : | IL12A & IL12B-1504M |
Product Overview : | Mouse IL12A (P43431) (Met 1-Ala 215) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1. Mouse IL12B (P43432) (Met 1-Ser 335) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed in human cells and purified. |
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Source : | HEK293 |
Species : | Mouse |
Tag : | Fc |
Form : | Lyophilized Powder |
Bio-activity : | 1. Immobilized mouse mIL12A+mIL12B at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His, The EC50 of biotinylated human IL12RB1-His is 38.7-90.3 ng/ml. 2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.2-0.8 ng/mL. |
Molecular Mass : | The recombinant mouse IL12 heterodimer of IL12A/IL12B, both was Fc chimera, comprises 988 (434 + 554) amino acids and has a calculated molecular mass of 111.5 (48.7 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 75 kDa respectively in SDS-PAGE under reducing conditions. |
Purity : | > 85 % as determined by SDS-PAGE |
Storage : | Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Storage buffer : | PBS, pH 7.4 |
Official Symbol : | IL12A & IL12B |
Synonyms : | IL12A; IL12B |
Official Symbol : | IL12A & IL12B |
Synonyms : | IL12A; IL12B |
Products Types
◆ Recombinant Protein | ||
IL12A & IL12B-1172M | Recombinant Mouse IL12A & IL12B protein(Met1-Ala215 & Met1-Ser335), His&hFc-tagged | +Inquiry |
◆ Lysates | ||
IL12A & IL12B-1780MCL | Recombinant Mouse IL12A & IL12B Overexpression Lysate(Met 1-Ala 215&Met 1-Ser 335) | +Inquiry |
IL12A & IL12B-1782MCL | Recombinant Mouse IL12A & IL12B Overexpression Lysate(Met 1-Ala 215&Met 1-Ser 335) | +Inquiry |
IL12A & IL12B-1779MCL | Recombinant Mouse IL12A & IL12B Overexpression Lysate(Met 1-Ala 215&Met 1-Ser 335) | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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