Recombinant Mouse IL12A & IL12B protein(Met1-Ala215 & Met1-Ser335), His&hFc-tagged
Cat.No. : | IL12A & IL12B-1172M |
Product Overview : | Recombinant Mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with the Fc region of Human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified in HEK293. |
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Source : | HEK293 |
Species : | Mouse |
Tag : | C-His&hFc |
Protein length : | Met1-Ala215 & Met1-Ser335 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its ability to mouse IL12RB2-His in a functional ELISA. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 15-90 ng/mL. |
Molecular Mass : | The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12B is Fc chimera, comprises 758 (204 + 554) amino acids and has a calculated molecular mass of 85.9 (23.1 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 75 kDa respectively in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 85 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | Il12a interleukin 12a [ Mus musculus ] |
Official Symbol : | IL12A |
Synonyms : | IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232; |
Gene ID : | 16159 |
mRNA Refseq : | NM_001159424 |
Protein Refseq : | NP_001152896 |
Products Types
◆ Recombinant Protein | ||
IL12A & IL12B-1504M | Active Recombinant Mouse IL12A & IL12B Heterodimer Protein, Fc-tagged | +Inquiry |
◆ Lysates | ||
IL12A & IL12B-1782MCL | Recombinant Mouse IL12A & IL12B Overexpression Lysate(Met 1-Ala 215&Met 1-Ser 335) | +Inquiry |
IL12A & IL12B-1781MCL | Recombinant Mouse IL12A & IL12B Overexpression Lysate(Met 1-Ala 215&Met 1-Ser 335) | +Inquiry |
IL12A & IL12B-1779MCL | Recombinant Mouse IL12A & IL12B Overexpression Lysate(Met 1-Ala 215&Met 1-Ser 335) | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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